159,258 results

1. VRK1-dTag-puro

   Plasmid

   #199651

   Purpose

   Expresses VRK1 C-term. Tagged with dTAG

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   dTAG

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PfICH pET15b

   Plasmid

   #60684

   Purpose

   Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

   Insert

   inhA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-Archon1-KGC-EGFP-ER2-WPRE

   Plasmid

   #108423

   Purpose

   Archon1 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PS-CFP2-TBRII-N-18

   Plasmid

   #57249

   Purpose

   Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   TBRII (TGFBR2 Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. lentiGuideFE-Puro

   Plasmid

   #170069

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   Cas9 guide RNA scaffold with the F+E scaffold modification
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

   Plasmid

   #234846

   Purpose

   3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

   Depositor

   Malin Parmar

   Article

   Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IP-EGFP-PA-BAX(ER)

   Plasmid

   #228941

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMRX-IP-EGFP-LOV2-Cyb5a

   Plasmid

   #233348

   Purpose

   Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted (Cyb5a 95-134) and N538E

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRX-IZ-SEC61B-mCherry-FRB

   Plasmid

   #233349

   Purpose

   Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   SEC61B (SEC61B Human)
   mCherry
   FRB
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   T2098L mutant of FKBP-rapamycin binding domain of…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRX-IB-FKBP-HaloTag

    Plasmid

    #233350

    Purpose

    Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Insert

    FKBP
    

    Use

    Retroviral

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAIP4_3KB5

    Plasmid

    #234225

    Purpose

    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KB5 (TRIM72 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRDB_270

    Plasmid

    #231111

    Purpose

    C>T base editor

    Depositor

    John Doench, David Root

    Insert

    TadA-CDd-nCas9-SpG-UGI-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4_NFAT-RE-CMVmin-BC0538-luc2

    Plasmid

    #227140

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC05038

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MEK1-E102-I103del-V5

    Plasmid

    #217301

    Purpose

    Lentivirus vector for expression of MEK1 E102-I103deletion

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    E102-I103deletion

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

    Plasmid

    #211968

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge