176,131 results

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Lenti_MCP-LSD1_Hygro

Plasmid

#138457

Purpose

lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP2

Plasmid

#141256

Purpose

Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Publication

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Venus

Plasmid

#127346

Purpose

Expresses Venus constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Venus Fluorescent Protein

Expression

Mammalian

Promoter

CAGGS Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAVS1-TRE-MA4-GFP

Plasmid

#114380

Purpose

Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

Depositor

Yuet Wai Kan

Publication

Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

MLL-AF4 fusion gene (AFF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

TRE

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-AT

Plasmid

#133306

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Publication

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR221_SLC1A5

Plasmid

#131974

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC1A5 (SLC1A5 Human)

Expression

Mammalian

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pORANGE Gria2-GFP KI

Plasmid

#131490

Purpose

Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria2, Gria2 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-Turbo-YFP-NLS-STOP-L2

Plasmid

#127361

Purpose

gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NLS, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD1-EGFP

Plasmid

#131206

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Publication

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD1 (NOD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR4_G3BP1

Plasmid

#127104

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Use

Gateway Cloning

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Publication

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRZero

Plasmid

#120275

Purpose

Blunt-end cloning vector of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

Plasmid

#125256

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

Depositor

Roger D. Kamm

Publication

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2(H134R)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a-forward

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PCDNA3-GFP1-9 T2A mCherry

Plasmid

#124430

Purpose

Expresses GFP1-9 and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Publication

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9 T2A mCherry

Expression

Mammalian

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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negMaLionG

Plasmid

#113907

Purpose

negative control for Green ATP sensor

Depositor

Tetsuya Kitaguchi

Publication

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionG

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-COX4-dAPEX2

Plasmid

#117176

Purpose

Plasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHAGE-KRAS-G12V

Plasmid

#116424

Purpose

Lentiviral expression of KRAS G12V

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Publication

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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