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Depositing Lab
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Depositing OrganizationSt. Jude Children's Research Hospital
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 28175-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 28175-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-control vector
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5256
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuciferase flanked by tp53 UTRs
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SpeciesH. sapiens (human)
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Insert Size (bp)1352
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MutationNote that insert length is based on sum of the 2 UTRs (145+1207)
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII/NcoI (5'UTR) (not destroyed)
- 3′ cloning site XbaI (3'UTR) (not destroyed)
- 5′ sequencing primer pGL3ctrl luc spb1
- 3′ sequencing primer pGL3 spb2
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 28175-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 28175-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
145-pGL3ctrl-3' UTR was a gift from Michael Kastan (Addgene plasmid # 28175 ; http://n2t.net/addgene:28175 ; RRID:Addgene_28175) -
For your References section:
5'-3'-UTR interactions regulate p53 mRNA translation and provide a target for modulating p53 induction after DNA damage. Chen J, Kastan MB. Genes Dev. 2010 Oct 1. 24(19):2146-56. 10.1101/gad.1968910 PubMed 20837656
Map uploaded by Addgene staff.
