Purpose(Empty Backbone) T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26536||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3240
Vector typeT/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth instructionsGrow in invitrogen DB3.1 cells in presence of kanamycin and Chloramphenicol
Copy numberHigh Copy
Insert Size (bp)1600
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer SP6 (Common Sequencing Primers)
For cloning taq polymerase-amplified PCR products, digest vector with Eam1105I, purify, ligate with PCR product, and use to transform DH5-alpha cells (or strain of choice). dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJC53.2 was a gift from Phillip Newmark (Addgene plasmid # 26536)
For your References section:Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, Sweedler JV, Newmark PA. PLoS Biol. 2010 . 8(10):e1000509. 10.1371/journal.pbio.1000509 PubMed 20967238