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pJC53.2
(Plasmid #26536)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26536 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJC53.2
  • Backbone size (bp) 3240
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    Grow in invitrogen DB3.1 cells in presence of kanamycin and Chloramphenicol
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Insert Size (bp)
    1600

Cloning Information

Resource Information

Depositor Comments

For cloning taq polymerase-amplified PCR products, digest vector with Eam1105I, purify, ligate with PCR product, and use to transform DH5-alpha cells (or strain of choice). dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC53.2 was a gift from Phillip Newmark (Addgene plasmid # 26536)
  • For your References section:

    Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, Sweedler JV, Newmark PA. PLoS Biol. 2010 . 8(10):e1000509. 10.1371/journal.pbio.1000509 PubMed 20967238