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pTRE_tau-LacZ::tTA-H100Y
(Plasmid #34927)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34927 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tau
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    4274
  • Entrez Gene
    MAPT (a.k.a. tau)
  • Promoter tetO
  • Tag / Fusion Protein
    • LacZ (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer LacZ-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tTA
  • Insert Size (bp)
    468
  • Mutation
    Made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain
  • Promoter tetO

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer BGH_rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Contains a bi-cistronic tetO promoter that drives expression of tau-LacZ, as well as a tTA-H100Y that was made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain.

Also known as pAuto5.

Addgene NGS QC analysis identified a 54 bp deletion in the tau open reading frame, relative to the provided sequence from the depositing laboratory. Based on these NGS results, the tau ORF shows 100% identity to amino acids 1-382 of tau isoform X14 [Bos taurus] XP_024835348.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE_tau-LacZ::tTA-H100Y was a gift from Mark Mayford (Addgene plasmid # 34927 ; http://n2t.net/addgene:34927 ; RRID:Addgene_34927)
  • For your References section:

    Localization of a stable neural correlate of associative memory. Reijmers LG, Perkins BL, Matsuo N, Mayford M. Science. 2007 Aug 31;317(5842):1230-3. 10.1126/science.1143839 PubMed 17761885