pTRE_tau-LacZ::tTA-H100Y
(Plasmid #34927)

• Depositing Lab: Mark Mayford
• Publication: Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

Backbone

• Vector backbone: N/A
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: tau
• Species: B. taurus (bovine)
• Insert Size (bp): 4274
• Entrez Gene: MAPT (a.k.a. tau)
• Promoter: tetO
• Tag / Fusion Protein:
  • LacZ (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: LacZ-R

Gene/Insert 2

• Gene/Insert name: tTA
• Insert Size (bp): 468
• Mutation: Made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain
• Promoter: tetO

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: BGH_rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a bi-cistronic tetO promoter that drives expression of tau-LacZ, as well as a tTA-H100Y that was made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain.

Also known as pAuto5.

Addgene NGS QC analysis identified a 54 bp deletion in the tau open reading frame, relative to the provided sequence from the depositing laboratory. Based on these NGS results, the tau ORF shows 100% identity to amino acids 1-382 of tau isoform X14 [Bos taurus] XP_024835348.1.