pTRE_tau-LacZ::tTA-H100Y
(Plasmid #34927)

• Depositing Lab: Mark Mayford
• Depositing Organization: Scripps California (The Scripps Research Institute)
  Located in the United States of America
• Publication: Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.
• Citations: 1 Article

Backbone

• Vector backbone: N/A
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: tau
• Species: B. taurus (bovine)
• Insert Size (bp): 4274
• Entrez Gene: MAPT (a.k.a. tau)
• Promoter: tetO
• Tag / Fusion Protein:
  • LacZ (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: LacZ-R

Gene/Insert 2

• Gene/Insert name: tTA
• Insert Size (bp): 468
• Mutation: Made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain
• Promoter: tetO

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: BGH_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a bi-cistronic tetO promoter that drives expression of tau-LacZ, as well as a tTA-H100Y that was made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain.

Also known as pAuto5.

Addgene NGS QC analysis identified a 54 bp deletion in the tau open reading frame, relative to the provided sequence from the depositing laboratory. Based on these NGS results, the tau ORF shows 100% identity to amino acids 1-382 of tau isoform X14 [Bos taurus] XP_024835348.1.

DNA (Catalog # 34927-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 34927-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.