Engineered calcium-precipitable restriction enzyme.
Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, Zimmermann SA, Brasino M, Rokicki J, Dowell RD
ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m. PubMed Article
Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, Zimmermann SA, Brasino M, Rokicki J, Dowell RD
ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85165 | pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP | Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation. |
| 85166 | pSB1A3 EcoRI Methylase-AmilCP | For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression. |