We narrowed to 2,200 results for: dcas9

1. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO-FUW-VdC9BV

   Plasmid

   #62195

   Purpose

   Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

   Depositor

   Kam Leong

   Article

   Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

   Insert

   VP64dCas9BFPVP64
   

   Use

   Lentiviral

   Tags

   Two VP64s tagged to dCas9 fused to BFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive) Cas9 (dCas9)

   Promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR1063

   Plasmid

   #111091

   Purpose

   Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   dCas9-KRAB
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAPH021

   Plasmid

   #234346

   Purpose

   Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

   Depositor

   Jerome Fox

   Article

   Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

   Inserts

   Type II dCas9 sgRNA
   Type II dCas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. THI ONLY pALD4 T-

   Plasmid

   #161497

   Purpose

   Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2 (non activation control NAC)
   gRNA (Non targeting control NTC)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK434

   Plasmid

   #155378

   Purpose

   PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A4NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Promoter

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPathBrick

   Plasmid

   #65006

   Purpose

   E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive native promoters

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYDE009

   Plasmid

   #194151

   Purpose

   Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   tetR-tetP-dcas9-rrnB T1-T7Te
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetp

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLJR965

    Plasmid

    #115163

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPPC011

    Plasmid

    #171143

    Purpose

    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPPC001

    Plasmid

    #171138

    Purpose

    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPRi(v2)-Blast

    Plasmid

    #170068

    Purpose

    Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH0001

    Plasmid

    #85969

    Purpose

    UCOE-SFFV-dCas9-BFP-KRAB

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BFP-KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    SFFV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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