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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC293-P4B1

Plasmid

#203134

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC293

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC170-P2G2

Plasmid

#203011

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC170

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC170

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC157-P2F1

Plasmid

#202998

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC157

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC157

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC120-P2B12

Plasmid

#202961

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC120

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC120

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCIwt-PRM-Wasabi

Plasmid

#195553

Purpose

Always-on expression of mWasabi reporter with transcription activation by wildtype CI

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
CIwt

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_A

Plasmid

#185803

Purpose

A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(ABI1)+-86MP+erGFP

Expression

Plant

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC121-P2C1

Plasmid

#202962

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC121

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC121

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC282-P3H6

Plasmid

#203123

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC282

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC282

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC232-P3D4

Plasmid

#203073

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC232

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC232

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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