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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti.CMV/TO_PRKAA1

Plasmid

#74446

Purpose

Lentiviral expression vector encoding untagged WT human AMPK alpha 1

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

AMPK alpha 1 (PRKAA1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-Myc-AIM2

Plasmid

#73958

Purpose

Express c-Myc-tagged human AIM2 in mammalian cells

Depositor

Christian Stehlik

Publication

Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

Insert

absent in melanoma 2 (AIM2) (AIM2 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV iE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CAG-Peredox

Plasmid

#73807

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

Depositor

Gary Yellen

Publication

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

AAV

Expression

Mammalian

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQCXIP-F-luc

Plasmid

#73058

Purpose

Retroviral expression of firefly luciferase for in vivo imaging

Depositor

Michael Grusch

Insert

Firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-Y701F-STAT1

Plasmid

#71453

Purpose

Lentiviral expression of Y701F-STAT1

Depositor

George Stark

Publication

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Y701F

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1+ eiF4A2 myc HIS

Plasmid

#71658

Purpose

mammalian expression of eiF4A2

Depositor

Gideon Dreyfuss

Insert

EIF4A2 (EIF4A2 Human)

Tags

HIS and myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCDNA_Lifeact-GFP_NLS-mCherry

Plasmid

#69058

Purpose

Dual Fluorescent Reporter for F-actin and Nuclei

Depositor

Olivier Pertz

Insert

LifeAct-GFP_IRES_NLS-mCherry

Tags

Lifeact-GFP, NLS-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHBV-X/EnhI-Luc

Plasmid

#71418

Purpose

To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay

Depositor

Wang-Shick Ryu

Publication

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV X promoter and enhancer I driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV X

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SOX2se-CPG-TV

Plasmid

#70155

Purpose

a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

Depositor

Rudolf Jaenisch

Publication

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se

Use

Mouse Targeting and Synthetic Biology

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Publication

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

u6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Publication

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FFA1-Tango

Plasmid

#66280

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FFA1 (FFAR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
CCR2-Tango

Plasmid

#66239

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCR2 (CCR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CHRM1-Tango

Plasmid

#66248

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM1 (CHRM1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
OCT4-2A-mOrange

Plasmid

#66986

Purpose

Generate OCT4 mOrange reporter line without selection cassette

Depositor

Danwei Huangfu

Publication

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

2A-mOrange

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Publication

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Promoter

1700bp upstream of BLBP gene

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT-TODestRFP_RNAseH1

Plasmid

#65785

Purpose

expresses RFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Human, Zebrafish)

Tags

RFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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