167,150 results
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Plasmid#67882PurposePlant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter geneDepositorInsertuidA
ExpressionPlantMutationT120APromoterM24Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR
Plasmid#194925PurposemRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected hoDepositorTypeEmpty backboneUseCRISPRPromoterhPGKAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha 2xmcp HALO
Plasmid#64540PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.DepositorInsertMCP
UseLentiviralTagsHA, HALO, and NLSExpressionMammalianPromoterhuman ubiquitin C promoterAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1 F121A
Plasmid#99506PurposeLentiviral expression of mutant Becn1 leading to hyperactive autophagyDepositorAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDG
Plasmid#187440PurposeVSV-G envelope expressing plasmidDepositorInsertsExpressionMammalianAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-mpx:Lifeact-Ruby
Plasmid#45246DepositorInsertlifeact-ruby
UseZebrafish expressionPromotermpxAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2×PH(PLCδ)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
TagsGFPExpressionYeastMutationcontains aa11-140 of PLCδ (including the PH domai…PromoterPRC1Available SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
TagstdTomato-v5-GCaMP6fExpressionMammalianPromoterCMV Immediate EarlyAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorAvailable SinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0-6H-TEV-KRAS G12D
Plasmid#159439PurposeExpresses KRAS G12D in E.coliDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82706PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO Empty gRNA Expression Cassette
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
dTAG-IRF8
Plasmid#190620PurposeLentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradationDepositorInsertIRF8 (IRF8 Human)
UseLentiviralTagsFKBP12_G36VExpressionMammalianMutationsynonymous mutation for sgRNA resistance for sgIR…Available SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
Tags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 K222R
Plasmid#80887Purposemammalian expression of ALK7 K222RDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR3-CFP
Plasmid#13641DepositorAvailable SinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYAP1-3
Plasmid#193666PurposeTet inducible knockdown of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
BK beta1
Plasmid#113565Purposemouse BK beta1 subunit in mammalian expression vectorDepositorAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK297 (DHC1-mAID Hygro)
Plasmid#140542PurposeDHC1 tagging with mAIDDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR3DL2-Fc(DAPA)-AviTag-6xHis
Plasmid#156758PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertKIR3DL2 (KIR3DL2 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only