165,420 results

1. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pQhsCDtRNA-

   Plasmid

   #86015

   Purpose

   Multi-gene expression system with human box C/D proteins with U14

   Depositor

   Hong Li

   Article

   Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

   Inserts

   U14 (SNORD14A Human)
   15.5K (SNU13 Human)
   fibrillarin (FBL Human)
   NOP56 (NOP56 Human)
   NOP58 (NOP58 Human)
   

   Use

   Co-expression; purification

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   amino acids 1-401, amino acids 1-411, and amino a…

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ExRai-CKAR2

   Plasmid

   #236096

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-myc-Skp2

   Plasmid

   #19947

   Depositor

   Yue Xiong

   Article

   Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

   Insert

   S-phase kinase-associated protein 2 (p45) (SKP2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDESTmycYBX1

   Plasmid

   #19878

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Y box binding protein 1 (YBX1 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK_Sox10_enh_84_Citrine

   Plasmid

   #130584

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_84
   

   Use

   Enhancer reporter

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA6.2EmGFP-DAXX

   Plasmid

   #169726

   Purpose

   Mammalian expression construct of GFP tagged full length DAXX

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX (DAXX Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MED12_pLX307

    Plasmid

    #98350

    Purpose

    Lentiviral expression of MED12

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MED12 (MED12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R1392Q

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LP725

    Plasmid

    #90182

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 561-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LP399

    Plasmid

    #90176

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1-1289

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. M13KO7-ΔPS-ΔgeneIII

    Plasmid

    #134351

    Purpose

    Helper phage for M13 phage production

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Helper phage gene
    

    Expression

    Bacterial

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALED_Right-RNR2-1397N

    Plasmid

    #183897

    Purpose

    Expresses TALED_Right-RNR2-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for RNR2 mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TALED_Left-RNR2-1397C-AD

    Plasmid

    #183896

    Purpose

    Expresses TALED_Left-RNR2-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for RNR2 mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

    Plasmid

    #172627

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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