176,159 results

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pJA42

Plasmid

#59930

Purpose

gRNA for cleavage at rol-6(su1006) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rol-6(su1006) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA-emerin-EGFP-polyA

Plasmid

#59747

Purpose

Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Alpha2-N-18

Plasmid

#55063

Depositor

Michael Davidson

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-JNKKTRmRuby2

Plasmid

#59148

Purpose

ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Tags

mRuby2

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-MyosinIIB-C-18

Plasmid

#55106

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

MyosinIIB (MYH10 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mT-Sapphire-pBAD

Plasmid

#54571

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

6xHis and mT-Sapphire

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-shp38

Plasmid

#52920

Purpose

expresses a hairpin specific for human p38MAPK

Depositor

Sheila Stewart

Publication

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pICSL11017 (pICH47732::NOSp-BAR-NOST)

Plasmid

#51145

Purpose

Confers Basta resistance in planta

Depositor

Jonathan D Jones

Insert

NOSp-BAR-NOST

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pST-KiT

Plasmid

#44562

Purpose

for integrative inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PML-3/pSG5

Plasmid

#50939

Purpose

expression of PML with Sumo sites destroyed

Depositor

Pier Pandolfi

Publication

Zhong et al Blood. 2000 May 1;95(9):2748-52.

Insert

PML-3 (PML Human)

Tags

HA

Expression

Mammalian

Mutation

K65R, K160R, K490R (Sumo modification sites)

Promoter

SV40

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Publication

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Publication

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pVITRO1-102.1F10-IgG4/λ

Plasmid

#50369

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Publication

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308D; S473D

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag-Jun4A-Myc

Plasmid

#47444

Purpose

mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

Depositor

Axel Behrens

Publication

Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

Insert

Jun 4A mutant (Jun Mouse)

Tags

Flag and myc

Expression

Mammalian

Mutation

four N-terminal residues phosphorylated by JNK, s…

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEV-G4-Km

Plasmid

#46819

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTD-CTwinStrep

Plasmid

#45939

Purpose

use to create C-terminal Twin-Strep-tag fusion proteins

Depositor

Thorben Dammeyer

Publication

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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