162,999 results

1. YFP-Parkin

   Plasmid

   #23955

   Purpose

   Mammalian expression of human Park2 fused to EYFP

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Park2 (PRKN Human)
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSP31

   Plasmid

   #199373

   Purpose

   Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Promoter

   Available since

   April 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

   Plasmid

   #84563

   Purpose

   Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
   

   Use

   Lentiviral

   Tags

   myc tag (9x)

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

   Plasmid

   #169188

   Purpose

   To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EMTB-mNeonGreen

   Plasmid

   #137802

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Ensconsin microtubule binding domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_ZBTB24_WT_V5

   Plasmid

   #83004

   Purpose

   Gateway Donor vector containing ZBTB24, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   ZBTB24 (ZBTB24 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_GPHN.FingR-eGFP-CCR5TC

   Plasmid

   #46296

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-eGFP-CCR5TC (GPHN Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX037

   Plasmid

   #167156

   Purpose

   MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   caffeic acid cycle genes
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pNOS, p35s

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQP-T2A

   Plasmid

   #102583

   Purpose

   Light-controlled activation of expression

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-VP16-T2A-NLS-GAL4-QPAS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF0930

    Plasmid

    #143242

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF83 (ZNF83 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-INU-FLAG-CCPG1

    Plasmid

    #128262

    Purpose

    Expresses CCPG1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    CCPG1 (CCPG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDHt-SK

    Plasmid

    #63615

    Purpose

    The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

    Depositor

    Seogchan Kang

    Article

    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

    Type

    Empty backbone

    Use

    Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

14. EK0396 BFPmut-stop-t70587 (pEAQ)

    Plasmid

    #191171

    Purpose

    EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t70587 (plant)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2242

    Plasmid

    #141986

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK647

    Plasmid

    #71701

    Purpose

    Produces E. coli thioredoxin reductase (EcTrxB)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti6-MK1-EHMT2-V5

    Plasmid

    #31113

    Depositor

    Bernard Futscher

    Insert

    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Stop codon mutated to Ala

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. multi-shRNA vector

    Plasmid

    #12391

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSin-NE1m

    Plasmid

    #172883

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    J. Julius Zhu

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Insert

    NE SENSOR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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