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Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
Liszczak G, Diehl KL, Dann GP, Muir TW
Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 10.1038/s41589-018-0097-1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111572pACEBac1-PARP1-Flag-His-WTExpresses full length PARP1 in Sf9 (insect) cells
111573pACEBac1-PARP1-Flag-His-7xKQExpresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
111574pACEBac1-PARP2-Flag-His-WTExpresses full length PARP2 in Sf9 (insect) cells
111575pCMV-PARP1-3xFlag-WTExpresses full length PARP1 in mammalian cells
111576pCMV-PARP1-3xFlag-7xKQExpresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells
111577pET30-HPF1-His-Sumo-FlagExpresses full length HPF1 in E. coli
111578pET30-ARH3-His-Sumo-HAExpresses ARH3 (amino acids 14-363) in E. coli

Antibodies from Article