162,999 results

1. pLenti6.2-Firefly-ccdB

   Plasmid

   #87077

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-phi29pol

   Plasmid

   #165573

   Purpose

   DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   phi29 DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Use

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SPORT6-RNF114-RINGmut

   Plasmid

   #69922

   Purpose

   Expresses mutant myc-RNF114 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C29A, C32A

   Promoter

   CMV

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. mEmerald-ERBB2-N-18

   Plasmid

   #62755

   Purpose

   Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMSCV-FlagMLL-pl-ENL(5613)

   Plasmid

   #20873

   Depositor

   Robert Slany

   Article

   Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

   Insert

   MLL (KMT2A, MLLT1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Plasmid

   #163058

   Purpose

   roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   roGFP2-Orp1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLAMP1-miRFP670nano3

   Plasmid

   #184669

   Purpose

   LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   LAMP1-miRFP670nano3
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad33_ISDge10_reRNA

   Plasmid

   #205340

   Purpose

   Bacterial expression of ISDge10 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISDge10 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PcDNA3.1-Flag

    Plasmid

    #210342

    Purpose

    Empty Vector for molecular cloning

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    Flag tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProEx N-Ras(G12C) CL

    Plasmid

    #224262

    Purpose

    Protein purification of N-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    N-Ras (NRAS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pProEx H-Ras(G12C) CL

    Plasmid

    #224261

    Purpose

    Protein purification of H-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCWXPGR-pTF-Wnt3a

    Plasmid

    #114287

    Purpose

    TET-inducible expression of Wnt3a

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-N BcRF1

    Plasmid

    #37924

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BcRF1 (BcRF1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLminP_Luc2P_RE31

    Plasmid

    #90373

    Purpose

    Hippo - TEAD1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hippo

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn5-145

    Plasmid

    #118529

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. miniCMV-EGFP-tDeg

    Plasmid

    #185400

    Purpose

    EGFP-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    EGFP-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-AR C619Y

    Plasmid

    #86429

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with point mutation C619Y

    Depositor

    Michael Mancini

    Article

    Nazareth et al Mol Endocrinol. 1999 Dec;13(12):2065-75.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C619Y

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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