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FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
PARP2-c098
Plasmid#216873PurposeBacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAST-2x attB
Plasmid#206362Purposeincludes two standard UAS cassettes and a third cassette without a promoterDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOV-VP16
Plasmid#42499PurposeLOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factorDepositorInsertFKF1 (N-LOV, G128D mutant) fused with VP16
UseTagsF-type VP16 activation domain and HA tagExpressionMammalianMutationG128D 1-168aaPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF3368
Plasmid#144844PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXR1 (FOXR1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0373
Plasmid#141594PurposeLentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFYA (NFYA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7
Plasmid#180315Purposemammalian expression of human SEPT7 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.V5.FLAG_NGFR
Plasmid#158250PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.V5.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Integrin β8-GFP
Plasmid#205093PurposeExpress GFP-tagged human integrin β8 in mammalian cellsDepositorInsertIntegrin beta 8 (ITGB8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro-KrasG12D2B
Plasmid#139786PurposeLentiviral tet-inducible expression of KrasG12D (mouse)DepositorInsertKrasG12D (Kras Mouse)
UseLentiviralTagsExpressionMammalianMutationG12DPromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG372
Plasmid#115602Purposesurface-displayed Nb1; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_H2A_C1
Plasmid#85051PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LPAR1-DuET
Plasmid#213329PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertLPAR1 (LPAR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HyperNova/pRSETB
Plasmid#221456PurposeFast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertHyperNova
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
