165,528 results

1. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

   Plasmid

   #232343

   Purpose

   Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF like 2 (HDGFL2 Human)
   

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINB-OTUB1*

   Plasmid

   #65441

   Purpose

   Expresses activated OTUB1 in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   UbcH5b (UBE2D2 Human)
   otubain-1 (OTUB1 )
   

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Active site cysteine mutated to alanine

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. R777-E214 Hs.RAF1-nostop

   Plasmid

   #70498

   Purpose

   Gateway ORF clone of human RAF1 [NM_002880.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

   Plasmid

   #62908

   Purpose

   Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-dVP16AD-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-Beta-Catenin-20

   Plasmid

   #54017

   Purpose

   Localization: Tight Junctions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Beta-Catenin (Ctnnb1 Mouse)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCDH-CMV-mCherry-EF1 Hygro

   Plasmid

   #129440

   Purpose

   express mCherry

   Depositor

   Oskar Laur

   Insert

   mcherry
   

   Use

   Lentiviral

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-CLIP170-N-18

   Plasmid

   #54044

   Purpose

   Localization: Microtubule Binding , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CLIP170 (CLIP1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. p6sheLLr

   Plasmid

   #37318

   Depositor

   John Schiller

   Article

   Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

   Insert

   HPV6L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTAMAHISTEV_PfeA

    Plasmid

    #128943

    Purpose

    expresses PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPRv2-sgFXN-1

    Plasmid

    #246017

    Purpose

    All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

    Depositor

    Vamsi Mootha

    Article

    Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

    Insert

    Frataxin (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv2-sgFXN-2

    Plasmid

    #246018

    Purpose

    All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

    Depositor

    Vamsi Mootha

    Article

    Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

    Insert

    Frataxin (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. NanoLuc-CCNE1 Vector

    Plasmid

    #238665

    Purpose

    Express NanoLuc-CCNE1 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CCNE1 (CCNE1 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CCNE1-NanoLuc Vector

    Plasmid

    #238666

    Purpose

    Express CCNE1-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CCNE1 (CCNE1 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. LDHA-HiBiT Vector

    Plasmid

    #238587

    Purpose

    Express LDHA-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    LDHA (LDHA Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #206860

    Purpose

    An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

    Depositor

    William Lagor

    Article

    De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

    Insert

    mLdlr gRNA (Ldlr Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1 LAMP1-mNeonGreen HRD

    Plasmid

    #207549

    Purpose

    Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible LAMP1-mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL20_mEGFP_WDR37_JAK2

    Plasmid

    #205954

    Purpose

    Express mEGFP-tagged fusion protein, WDR37_JAK2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    WDR37_JAK2 (WDR37, JAK2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CL20_mEGFP_DDX3X_MRAS

    Plasmid

    #205803

    Purpose

    Express mEGFP-tagged fusion protein, DDX3X_MRAS from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    DDX3X_MRAS (DDX3X, MRAS Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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