Fibronectin-human-EDA in pMAX
(Plasmid #120402)

• Purpose: enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence
• Depositing Lab: Inaam Nakchbandi
• Publication: Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. doi: 10.1371/journal.pbio.1002562. eCollection 2016 Sep.

Backbone

• Vector backbone: pMAX-Cloning
• Backbone size w/o insert (bp): 2868
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EDA-fibronectin
• Alt name: Human fibronectin containing complete variable region and EDA (or EIIIA) domain
• Alt name: EIIIA fibronectin, cellular fibronectin
• Species: H. sapiens (human)
• Insert Size (bp): 7184
• Entrez Gene: FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone EDA sp44. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms.

See also "Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 2017 May 12;292(19):7745-7760." https://www.ncbi.nlm.nih.gov/pubmed/28325836