Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction.
Steinhorn B, Sorrentino A, Badole S, Bogdanova Y, Belousov V, Michel T
Nat Commun. 2018 Oct 2;9(1):4044. doi: 10.1038/s41467-018-06533-2. PubMed Article
Steinhorn B, Sorrentino A, Badole S, Bogdanova Y, Belousov V, Michel T
Nat Commun. 2018 Oct 2;9(1):4044. doi: 10.1038/s41467-018-06533-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119164 | pAAV-HyPer-DAAO-NES | Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter. |
| 119183 | pAAV-HyPer3 | AAV expression vector with CMV promoter driving expression of the hydrogen peroxide-sensitive fluorescent biosensor HyPer3. |
| 119184 | pAAV-SypHer2-DAAO-NES | Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer. |