165,528 results

1. pFA6a-6xGLY-FLAG-kanMX6

   Plasmid

   #20751

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-iG

   Plasmid

   #27358

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   U6 promoter, SFFV promoter, IRES-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Neo-Bam APC 1-1309

   Plasmid

   #16509

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 1309 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pMRE-Tn7-161

   Plasmid

   #118573

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-FHH-Empty Vector-IRES-Puro

   Plasmid

   #120568

   Purpose

   For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag-HA-6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-Cas9

   Plasmid

   #166601

   Purpose

   Encodes Cre-dependent Cas9 under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   Cas9
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG/III-hs-MBP-CB2-HF

   Plasmid

   #121682

   Purpose

   Bacterial expression of cannabinoid receptor CB2 fusion protein

   Depositor

   Richard Henderson, Reinhard Grisshammer

   Article

   Calandra et al Biotechnology Letters

   Insert

   peripheral cannabinoid receptor (CNR2 Human)
   

   Tags

   His6 Flag tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST_EWSR1

   Plasmid

   #26377

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p146

    Plasmid

    #186178

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 146
    

    Use

    Synthetic Biology; Cloning

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTOM20-ddGFP-B

    Plasmid

    #40291

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    ddGFP-B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3XFlag DVL2 (WT)

    Plasmid

    #24802

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV uN2C GFP (Ctl)

    Plasmid

    #224355

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intyron (CAG)

    Available Since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid

    #78174

    Purpose

    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEGFP-Y705F-STAT3

    Plasmid

    #71445

    Purpose

    Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Mutation

    Y705F

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

18. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV/TO Neo empty (w215-1)

    Plasmid

    #17485

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. phCMV-229E-D15

    Plasmid

    #188908

    Purpose

    express truncated 229E spike for pseudovirus production

    Depositor

    David Nemazee

    Insert

    229E spike (S 229E)
    

    Expression

    Mammalian

    Mutation

    c-terminal 15aa deletion

    Promoter

    CMV

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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