162,999 results

1. pDARMO_CMVT_3xFLAG_BIRC6

   Plasmid

   #197967

   Purpose

   Mammalian cell expression of wt 3xFLAG-BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L1332V

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-hSOX2

   Plasmid

   #17218

   Purpose

   Retroviral expression of human SOX2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 18 pCOLI_A_Trx

   Plasmid

   #160255

   Purpose

   N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   6xHis and Thioredoxin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4.0-TERT G228A

   Plasmid

   #84926

   Purpose

   luciferase reporter for mutant TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   G228A

   Promoter

   TERT

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-CAG-IntCCCre-T2A-CopGFP

   Plasmid

   #160501

   Purpose

   Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   CopGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJet1.2 ermB cassette

   Plasmid

   #117121

   Purpose

   Erythromycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Erythromycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAVS1-P-CAG-mCh

   Plasmid

   #80492

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   mCherry
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Opie2-PuroR

   Plasmid

   #131613

   Purpose

   Expresses PuroR and dsRed.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR
   dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Opie2 and hr5Ie1

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Green Glifon4000

    Plasmid

    #126208

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon4000
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. synIRES_RC25

    Plasmid

    #189970

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    synIRES_RC25
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUltra-hot

    Plasmid

    #24130

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

    Depositor

    Malcolm Moore

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSIIpuro-EGF-ScNeo

    Plasmid

    #209893

    Purpose

    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EGF-ScNeo (EGF Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCSIIpuro-HBEGF-ScNeo

    Plasmid

    #209894

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LgBiT-ST

    Plasmid

    #207633

    Purpose

    A plasmid encoding LgBiT fused to SpyTag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    LgBiT-SpyTag
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. V51 pIRESpuro-GLUE (pGLUE)

    Plasmid

    #15100

    Purpose

    tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Type

    Empty backbone

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF2259

    Plasmid

    #141997

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX13 (SOX13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge