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PurposeExpresses myc tagged mouse reelin ORF in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCrl
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Backbone manufacturerCurran lab (Addgene plasmid 122443)
- Total vector size (bp) 16000
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Modifications to backboneMyc epitope tag 9E10 (QKLISEEDLN) was inserted into the unique BspEI site present in pCrl
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namereelin
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SpeciesM. musculus (mouse)
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Insert Size (bp)10383
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GenBank IDU24703.1
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Entrez GeneReln (a.k.a. reeler, rl)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCrlM was a gift from Tom Curran (Addgene plasmid # 122444 ; http://n2t.net/addgene:122444 ; RRID:Addgene_122444) -
For your References section:
Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T. J Neurosci. 1997 Jan 1;17(1):23-31. PubMed 8987733
