Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.
Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5. PubMed

Plasmids from Article

ID Plasmid Purpose
105937pCS2 PCNA GFPin vitro transcription, cell cycle nuclear marker
105978p3E LAP2b3' element for Gateway cloning, lamina-associated polypeptide 2
105979bactin:GFP-PCNAtransgenesis, ubiquitous expression
105980bactin:GFP-LAP2btransgenesis, ubiquitous expression
117699pCAGGs-NLS-TIR1_P2A_NES-TIR1Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
117713pCDNA5FRT/TO_HA-mAID-nanobodyExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)
117714pIREShygro HA-mVenus-mAIDExpression of HA-tagged mVenus fused to mAID
117716pCS2+_NLS-Tir1-HAmRNA synthesis of NLS-TIR1-HA for injection
117717pCS2+_Flag-myc-NES-Tir1mRNA synthesis of Flag-myc-NES-TIR1 for injection
117718pCS2+_HA-mAID-nanobody (Kless)mRNA synthesis of lysine-less HA-mAID-nanobdy for injection
117720pIRES_IBB-mCherry-IRES-HA-mAID-nanobodyExpression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

Antibodies from Article