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Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.
Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
105937pCS2 PCNA GFPin vitro transcription, cell cycle nuclear marker
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105978p3E LAP2b3' element for Gateway cloning, lamina-associated polypeptide 2
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105979bactin:GFP-PCNAtransgenesis, ubiquitous expression
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105980bactin:GFP-LAP2btransgenesis, ubiquitous expression
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117699pCAGGs-NLS-TIR1_P2A_NES-TIR1Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
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117713pCDNA5FRT/TO_HA-mAID-nanobodyExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)
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117714pIREShygro HA-mVenus-mAIDExpression of HA-tagged mVenus fused to mAID
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117716pCS2+_NLS-Tir1-HAmRNA synthesis of NLS-TIR1-HA for injection
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117717pCS2+_Flag-myc-NES-Tir1mRNA synthesis of Flag-myc-NES-TIR1 for injection
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117718pCS2+_HA-mAID-nanobody (Kless)mRNA synthesis of lysine-less HA-mAID-nanobdy for injection
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117720pIRES_IBB-mCherry-IRES-HA-mAID-nanobodyExpression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA
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Antibodies from Article