165,528 results

1. pCDH-3_Flag-YTHDF2

   Plasmid

   #238975

   Purpose

   Expresses YTHDF2

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8138-E02

   Plasmid

   #225659

   Purpose

   Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   SORE6
   

   Use

   Gateway entry clone

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 BMPS WT-TEV-6xHis

   Plasmid

   #204837

   Purpose

   Mammalian expression of recombinant BMPS WT

   Depositor

   Monther Abu-Remaileh

   Article

   Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

   Insert

   CLN5 (CLN5 Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_EF1a_Gag-EIF4E-TM

   Plasmid

   #212658

   Purpose

   piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Piggybac

   Tags

   EIF4E (W56L, W102L, W166L)

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT_S1-H1

   Plasmid

   #218392

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MUT_S1-H1

   Plasmid

   #218394

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCR-YFP-GW

    Plasmid

    #209366

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWOL-YFP-GW

    Plasmid

    #209365

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-eIF4E-TwStrep

    Plasmid

    #208638

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

    Depositor

    Quanfu Ma

    Insert

    human eIF4E (ENST00000450253) (EIF4E Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.4_1G11F2-3 heavy chain

    Plasmid

    #198253

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-HseIF4AI_T

    Plasmid

    #147543

    Purpose

    Insect Expression of HseIF4AI

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4AI (EIF4A1 Human)
    

    Expression

    Insect

    Mutation

    3 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-EGFR-V5/HIS

    Plasmid

    #201102

    Purpose

    expression of human EGFR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKI_ΩBBMVP16&WUS

    Plasmid

    #220348

    Purpose

    Expresses BBM-VP16 and WUS in plant cells

    Depositor

    Tomoko Igawa

    Article

    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

    Inserts

    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    

    Tags

    VP16 transcriptional activation domain

    Expression

    Plant

    Promoter

    CaMV 35S and RPS5A

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-MMLV_RT (mut H)

    Plasmid

    #165546

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMXs-Nanog

    Plasmid

    #13354

    Purpose

    Retroviral expression of mouse Nanog

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. perbB2-EGFP

    Plasmid

    #39321

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Tags

    egfp

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. LD-RA

    Plasmid

    #209863

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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