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pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
(Plasmid #160540)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 160540 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pQE-30 and pcI-ts,ind+
  • Total vector size (bp) 9813
  • Modifications to backbone
    CmR gene from pQE-30 removed and AmpR from pcI-ts,ind+ removed.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth at 37 or addition of nalidixic acid induces expression of NSP7 and NSP8
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SARS-CoV-2 NSP7
  • Alt name
    Severe acute respiratory syndrome coronavirus 2 non-structural protein 7
  • Species
    Synthetic
  • Insert Size (bp)
    255
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Gene/Insert 2

  • Gene/Insert name
    SARS-CoV-2 NSP8
  • Alt name
    Severe acute respiratory syndrome coronavirus 2 non-structural protein 8
  • Species
    Synthetic
  • Insert Size (bp)
    600
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Gene/Insert 3

  • Gene/Insert name
    SARS-CoV-2 NSP12
  • Alt name
    Severe acute respiratory syndrome coronavirus 2 non-structural protein 12
  • Species
    Synthetic
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized NSP7, NSP8, and NSP12 genes were from custom plasmids synthesized by Genscript.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8) was a gift from Kenneth Johnson (Addgene plasmid # 160540 ; http://n2t.net/addgene:160540 ; RRID:Addgene_160540)
  • For your References section:

    Remdesivir Is Effective in Combating COVID-19 because It Is a Better Substrate than ATP for the Viral RNA-Dependent RNA Polymerase. Dangerfield TL, Huang NZ, Johnson KA. iScience. 2020 Nov 28:101849. doi: 10.1016/j.isci.2020.101849. 10.1016/j.isci.2020.101849 PubMed 33283177