Nuclear sensing of breaks in mitochondrial DNA enhances immune surveillance.
Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w. Epub 2021 Feb 24. PubMed Article
Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w. Epub 2021 Feb 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167288 | pLenti-RIGI-Apex- T2A-EGFP | Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor. |
| 167289 | pTRIP-SFFV-mtagBFP-2A RIGI WT | Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I |
| 167290 | pTRIP-SFFV-mtagBFP-2A RIGI K270A | Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I |
| 167293 | pLKO.1 puro_shDDX58_230212 | Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette. |
| 167294 | pLKO.1 puro_shTBK1_3185 | Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette. |
| 167295 | pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2 | Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry |
| 167296 | pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2 | Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP |