An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes.
Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. PubMed Article
Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198383 | pLX304 Flag-mCherry-eEF1A1_IRES-GFP | eEF1A1 fluorescent reporter |
| 198384 | pHR V5-RNF14_IRES-mCherry | RNF14 expression |
| 198385 | pHR V5-RNF14 C417A_IRES-mCherry | RNF14 C417A expression |
| 198386 | pHR V5-RNF25_IRES-mCherry | RNF25 expression |
| 198387 | pHR V5-RNF25 dRING_IRES-mCherry | RNF25 (?RING) expression |
| 198388 | pLenti6.3 GCN1-3xFlag_IRES-iRFP | GCN1 expression |
| 198389 | pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP | GCN1 R2312A expresson |
| 198390 | pHR RPS27A-3xFlag_IRES-GFP | RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression |
| 198391 | pHR RPS27A K113R-3xFlag_IRES-GFP | RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression |
| 198392 | pHR RPS27A K107/113R-3xFlag_IRES-GFP | RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression |
| 198393 | pLX304 3xFlag-eRF1_IRES-GFP | eRF1 expression |
| 198394 | pLX304 3xFlag-eRF1 G183/184A_IRES-GFP | eRF1 (AAQ mutant) expression |