Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.
Massengill CI, Bayless-Edwards L, Ceballos CC, Cebul ER, Cahill J, Bharadwaj A, Wilson E, Qin M, Whorton MR, Baconguis I, Ye B, Mao T, Zhong H
Nat Methods. 2022 Nov;19(11):1461-1471. doi: 10.1038/s41592-022-01646-5. Epub 2022 Oct 27. PubMed Article
Massengill CI, Bayless-Edwards L, Ceballos CC, Cebul ER, Cahill J, Bharadwaj A, Wilson E, Qin M, Whorton MR, Baconguis I, Ye B, Mao T, Zhong H
Nat Methods. 2022 Nov;19(11):1461-1471. doi: 10.1038/s41592-022-01646-5. Epub 2022 Oct 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182279 | pCDNA3_cAMPFIRE-L | Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor. |
| 182280 | pCDNA3_cAMPFIRE-M | Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor. |
| 182281 | pCDNA3_cAMPFIRE-H | Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor. |
| 182282 | pCDNA3_cAMPFIRE-L-R279E | Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor. |
| 182283 | pCAGGS_cAMPFIRE-L | Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer. |
| 182284 | pCAGGS_cAMPFIRE-M | Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer. |
| 182285 | pCAGGS_cAMPFIRE-H | Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer. |
| 182286 | pCAGGS_cAMPFIRE-L-R279E | Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer. |