We narrowed to 9,915 results for: pET

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pET-6xHis-GFP(0)-L1

Plasmid

#205033

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Publication

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(0)-L1

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, I138R, D207R, N222K, L246R

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+24)

Plasmid

#199163

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+24)

Tags

MGHHHHHHGGA

Mutation

E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2_rpoA-U

Plasmid

#190956

Purpose

Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Publication

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-PLS-KP3_rpoA-U

Plasmid

#190960

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Publication

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET - FnR062.Y78W

Plasmid

#84802

Purpose

bacterial expression of FnR.0.6.2-Y78W protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78W

Tags

6xHis

Expression

Bacterial

Mutation

Y78W

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78D

Plasmid

#84786

Purpose

bacterial expression of FnR.0.6.2-Y78D protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78D

Tags

6xHis

Expression

Bacterial

Mutation

Y78D

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78F

Plasmid

#84788

Purpose

bacterial expression of FnR.0.6.2-Y78F protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78F

Tags

6xHis

Expression

Bacterial

Mutation

Y78F

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78G

Plasmid

#84789

Purpose

bacterial expression of FnR.0.6.2-Y78G protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78G

Tags

6xHis

Expression

Bacterial

Mutation

Y78G

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78H

Plasmid

#84790

Purpose

bacterial expression of FnR.0.6.2-Y78H protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78H

Tags

6xHis

Expression

Bacterial

Mutation

Y78H

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78I

Plasmid

#84791

Purpose

bacterial expression of FnR.0.6.2-Y78I protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78I

Tags

6xHis

Expression

Bacterial

Mutation

Y78I

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78P

Plasmid

#84796

Purpose

bacterial expression of FnR.0.6.2-Y78P protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78P

Tags

6xHis

Expression

Bacterial

Mutation

Y78P

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78R

Plasmid

#84798

Purpose

bacterial expression of FnR.0.6.2-Y78R protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78R

Tags

6xHis

Expression

Bacterial

Mutation

Y78R

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78S

Plasmid

#84799

Purpose

bacterial expression of FnR.0.6.2-Y78S protein

Depositor

Benjamin Hackel

Publication

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78S

Tags

6xHis

Expression

Bacterial

Mutation

Y78S

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET-Cdc12-Cdc11

Plasmid

#131162

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Publication

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

6his-Cdc12 + Cdc11

Use

Bicistronic

Tags

Cdc11: S-Tag and Cdc12: 6his

Expression

Bacterial

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-eSpCas9

Plasmid

#126769

Purpose

Expression of increased fidelity eSpCas9 in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET sfGFP(150TAG)

Plasmid

#133455

Purpose

expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

Depositor

Yu-Hsuan Tsai

Publication

Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and mCherry

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MDC

Plasmid

#108950

Purpose

heterologous expression of MDC of S. cerevisiae in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate decarboxylase (MDC)

Tags

His6-Tag

Expression

Bacterial

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-PMK

Plasmid

#108949

Purpose

heterologous expression of PMK of E. faecalis in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Phosphomevalonate kinase (PMK)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-IDI2

Plasmid

#108951

Purpose

heterologous expression of IDI2 of T. thermophilus in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Isopentenyldiphosphat isomerase 2 (IDI2)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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