We narrowed to 3,219 results for: zebrafish

1. pVk7-33G1-Flag

   Plasmid

   #58950

   Purpose

   backbone for mouse recombinant antibody with added C-terminal FLAG tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-U6a-U6c-rac2 guide

   Plasmid

   #107600

   Purpose

   An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   rac2 guide RNA sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVk7-33G1-BioLHis

   Plasmid

   #58949

   Purpose

   backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   6His tag and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Tags

   Expression

   Mutation

   Promoter

   myo6b

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-mTagBFP

   Plasmid

   #75175

   Purpose

   TagBFP (blue) fluorophore with stop codon for C-terminal fusions

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   TagBFP
   

   Use

   Three prime entry vector for multisite gateway th…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hox D4a

   Plasmid

   #36469

   Depositor

   Robert Ho

   Article

   Prince et al Development. 1998 Feb . 125(3):407-20.

   Insert

   Hox D4a (hoxd4a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Bacterial

   Mutation

   Q100H, A101T, G125C and R189W

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSYC-97

   Plasmid

   #31565

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   pCS4+-NLS-EGFP-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-QUASM1

   Plasmid

   #155114

   Purpose

   5' entry vector containing QUASM1 promoter element.

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1
   

   Use

   Gateway 5' entry vector

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-H2B-mCerulean3

   Plasmid

   #75156

   Purpose

   Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

    Plasmid

    #171795

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-QUAS5x

    Plasmid

    #155113

    Purpose

    5' entry vector containing QUAS5x promoter element.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x
    

    Use

    Gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUAS5x-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-ngn1-Trpa1b-2A-mCherry

    Plasmid

    #106425

    Purpose

    For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pGEM-and1 in situ probe

    Plasmid

    #52102

    Purpose

    actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

    Depositor

    Didier Stainier

    Article

    Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

    Insert

    actinodin 1 in situ probe (and1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ MBIC5-mCer3

    Plasmid

    #74116

    Purpose

    For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

    Depositor

    Michael Tsang

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    MBIC5-mCer3
    

    Use

    Transgenic construct

    Tags

    mCerulean 3

    Expression

    Mutation

    Promoter

    Myl7 (Cmlc2)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-cryR;tmem88aCE1-P1Egfp

    Plasmid

    #90148

    Purpose

    contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    tmem88aE1P1Egfp (tmem88a Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-I-Sce1-slc6a3[11kb]:egfp

    Plasmid

    #53240

    Purpose

    allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT-GFP
    

    Use

    Cloning

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. actb2-lifeact-mKate2

    Plasmid

    #109750

    Purpose

    Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2 fused to the actin binding lifeact sequence
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-HybridBarcode

    Plasmid

    #174037

    Purpose

    For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    CRISPR target sites
    

    Use

    Tol2 transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAL3344

    Plasmid

    #42807

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-prothymosin alpha a-left (ptmaa Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only