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We narrowed to 4,636 results for: pcr

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Showing: 1601 - 1620 of 4636 results

  1. pECT1B

    Plasmid
    #75447
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1B
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75447-plasmid-map-sequence-id-146475

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  2. pECT1D

    Plasmid
    #75448
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1D
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75448-plasmid-map-sequence-id-146476

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  3. pECT1E

    Plasmid
    #75449
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1E
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75449-plasmid-map-sequence-id-146477

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  4. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid
    #202199
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Has Service
    AAV1 and AAV5
    Insert
    stChrimsonR, PdCO
    Use
    AAV and Cre/Lox
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Promoter
    hSyn1
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202199-plasmid-map-sequence-id-397078

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  7. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  9. pJL257

    Plasmid
    #243696
    Purpose
    A piggyBac vector containing the NDUFB9 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of NDUFB9 (NDUFB9 Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243696-plasmid-map-sequence-id-489271

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  13. pJL355

    Plasmid
    #243710
    Purpose
    A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    COX7A2 genomic locus, with its 3' UTR replaced by the ACTB 3' UTR (COX7A2 Human)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243710-plasmid-map-sequence-id-489295

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  15. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  19. pJL263

    Plasmid
    #243699
    Purpose
    A piggyBac vector containing the NDUFB9 genomic sequence fused with a mNeonGreen2(11) sequence, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    NDUFB9 (NDUFB9 Human)
    Tags
    mNeonGreen2(11)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243699-plasmid-map-sequence-id-489320

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  20. pJL347

    Plasmid
    #243707
    Purpose
    A piggyBac vector containing the COX5B coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of COX5B (COX5B Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243707-plasmid-map-sequence-id-489300

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Showing: 1601 - 1620 of 4636 results