We narrowed to 616 results for: multi

...developed a novel method for the easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless...Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single.... Number of fragments assembled simultaneously Multiple fragments can be assembled in one reaction. However...

...developed a novel method for the easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless...Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single.... Number of fragments assembled simultaneously Multiple fragments can be assembled in one reaction. However...

...Plasmid Kit Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral ...combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound...system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The ...imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or...

... in detail below [2]. Multipotency: When a cell can differentiate into multiple cell types of the same...[1]. Stem cells’ ability to differentiate into multiple cell types makes them useful models for developmental...hematopoietic) stem cells are a good example of multipotent cells. Unipotency: As the name suggests, cells...ASCs) Also known as somatic stem cells, these multipotent stem cells are located in small quantities in...

...increases. Multiple inserts: There are some cloning methods that make working with multiple inserts a ...as some methods are more amenable to combining multiple DNA fragments than others. In today’s post, we...per reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner...one right answer, so don’t be afraid to explore multiple approaches to find out which one works best for...

...REPAIR and RESCUE can function in a multiplexed manner to edit multiple RNAs. It’s clear that CRISPR RNA ...research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing systems...This change specifies the edit to be made when multiple As are present in the gRNA spacer, as ADAR will...As discussed above, the ability to edit RNA has multiple advantages, including reversibility and use in...

...resistance gene, allowing resistant bacteria to multiply, spreading their resistance genes throughout the...selects for resistant bacteria, allowing them to multiply and propagate their resistance genes. Our society...is hoping to combat antibiotic resistance using multiple approaches. Major restaurant chains including ...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

...overview of the MoClo assembly process to produce a multigene construct. Figure exemplifies the extraction and...and terminator flanking the CDS, and a Level 2 multigene vector with two other independently regulated ... or fluorescent markers? If you’re creating a multigene construct, in what position do you want your gene...important that these stay frozen at -80 °C, since multiple rounds of freezing and thawing will affect bacterial...plasmids with undesired ligations, so always screen multiple colonies after each transformation. This could...

...labs prefer to collect and pool the harvests from multiple time points while others opt for a single harvest...transfer and packaging plasmids. A benefit of the multiple harvest strategy is that each day you are supplying...destabilize the viral envelope. One drawback of the multiple harvest approach is that it tends to result in...that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

...mutations. Multiple gRNAs could be used to combat this problem, just as ART includes multiple drugs to ...transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal repeat (LTR...activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more ...

...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance...

...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance...

...within a narrower band of wavelengths to allow multiple tools, such as biosensors, to be used in tandem...receptors (GPCR) regulate cellular function through multiple intra-cellular signaling cascades. Optical methods...than traditional pharmacology techniques allow. Multiple photoswitches can be used in a single pathway ...optogenetic tool, it can also cause problems when using multiple optogenetics or biosensors in one system. There...Optogenetic tools provide a powerful way to study multiple aspects of cellular activity and physiology. However...

...genes. TIP: Addgene recommends that you select multiple target sequences for each gene. Some sequences...aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... SA et. al. RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient...

...genes. TIP: Addgene recommends that you select multiple target sequences for each gene. Some sequences...aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... SA et. al. RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient...

...smaller, easily customizable vector functions in multiple species, including tobacco and Arabidopsis. Improving...β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo et al....be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, ... if needed. In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing...

...through cloning. *Note — If you will be depositing multiple plasmids and would prefer to send us your plasmid...Expression vector. If your plasmid can be classified in multiple categories, please check all of the appropriate... can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...plasmids in your deposit. Pro Tip! If you have multiple plasmids with the same gene or vector backbone... Multiple Authors...

... Biosensor GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor... AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics... Multiple Authors...

...affect viral vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...rich media can improve growth and DNA yield. Test multiple, small colonies After streaking LB agar plates...cultures contain the recombined vector backbone. Test multiple colonies before saving a glycerol stock to ensure...try re-streaking for single colonies and testing multiple preps. In some cases, we suggest gel extraction...

...neuroscience experiments often rely on multiplexing, where multiple fluorescent tools are used simultaneously...or red sensors in multicolor experiments. With OCaMP, researchers can image multiple brain regions or ...