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We narrowed to 575 results for: multi

Showing: 181 - 200 of 575 results

  1. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...overview of the MoClo assembly process to produce a multigene construct. Figure exemplifies the extraction and...and terminator flanking the CDS, and a Level 2 multigene vector with two other independently regulated ... or fluorescent markers? If you’re creating a multigene construct, in what position do you want your gene...important that these stay frozen at -80 °C, since multiple rounds of freezing and thawing will affect bacterial...plasmids with undesired ligations, so always screen multiple colonies after each transformation. This could...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...labs prefer to collect and pool the harvests from multiple time points while others opt for a single harvest...transfer and packaging plasmids. A benefit of the multiple harvest strategy is that each day you are supplying...destabilize the viral envelope. One drawback of the multiple harvest approach is that it tends to result in...that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...mutations. Multiple gRNAs could be used to combat this problem, just as ART includes multiple drugs to ...transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal repeat (LTR...activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more ...

  4. Pipetting Protocol

    Type
    Protocol
    ...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...within a narrower band of wavelengths to allow multiple tools, such as biosensors, to be used in tandem...receptors (GPCR) regulate cellular function through multiple intra-cellular signaling cascades. Optical methods...than traditional pharmacology techniques allow. Multiple photoswitches can be used in a single pathway ...optogenetic tool, it can also cause problems when using multiple optogenetics or biosensors in one system. There...Optogenetic tools provide a powerful way to study multiple aspects of cellular activity and physiology. However...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...genes. TIP: Addgene recommends that you select multiple target sequences for each gene. Some sequences...aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... SA et. al. RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient...

  7. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...smaller, easily customizable vector functions in multiple species, including tobacco and Arabidopsis. Improving...β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo et al....be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, ... if needed. In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...through cloning. *Note — If you will be depositing multiple plasmids and would prefer to send us your plasmid...Expression vector. If your plasmid can be classified in multiple categories, please check all of the appropriate... can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...plasmids in your deposit. Pro Tip! If you have multiple plasmids with the same gene or vector backbone... Multiple Authors...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...affect viral vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...rich media can improve growth and DNA yield. Test multiple, small colonies After streaking LB agar plates...cultures contain the recombined vector backbone. Test multiple colonies before saving a glycerol stock to ensure...try re-streaking for single colonies and testing multiple preps. In some cases, we suggest gel extraction...

  10. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... Biosensor GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor... AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics... Multiple Authors...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...neuroscience experiments often rely on multiplexing, where multiple fluorescent tools are used simultaneously...or red sensors in multicolor experiments. With OCaMP, researchers can image multiple brain regions or ...

  13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...primer known as a megaprimer. Piecing together multiple PCR products using sequence homology is also common... Cloning, and SLIC can all be used to assemble multiple fragments, but these methods can be expensive ...is more expensive than gBlock synthesis, and multifragment assembly methods are preferred. No such restriction...other methods, such “BAC shaving” would require multiple steps. One potential pitfall of REPLACR-mutagenesis...

  14. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...vector-free cells Limited genomic integration Multiple transfections required Need to sequence to verify...-Free High High Transgene-free and vector-free Multiple transfections required Protein Delivery DNA-...kb, so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...to use a polycistronic cassette that expresses multiple reprogramming factors driven by one promoter. ...plasmid integration. Another challenge is that multiple transfection makes it difficult to control the...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for multiplexed targeting with Zhang lab plasmids? A6: For multiplexing CRISPR to target multiple genome...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science...-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated Genome Engineering...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...chance that the antibody could meet your needs.    Multiplex experiments Finally, consider whether you will... in the experiment. If you are planning to use multiple antibodies in a single experiment then you will...antibodies are sold under different names through multiple vendors, so look at epitope target, source, and...strategies that compare the staining patterns of multiple antibodies against the same target; tagged protein...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...interested in expressing multiple small genes, you can design single rAAV vectors with multiple genes packaged ...interested in heftier genes, you can coinfect with multiple viruses to express genes or gene sets over 4.7... Multiple Authors...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...currently available or being developed to treat a multitude of therapeutic indices, including hypertension...coherent manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator ...suggests that coactivators may be integrators of multiple cell signaling systems. Activation of membrane...expression patterns of nuclear receptors across multiple different tissues in normal and disease states...

  19. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer and...packaging systems divide the viral elements among multiple plasmids. Second generation packaging systems ...transfer gene. By dividing the viral elements across multiple plasmids, the risk of recombination and consequently...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...integrated into its genome and a cell that has had multiple integration events. In addition, promoter choice... cell’s genome. This approach can discriminate multiple integration events, reducing the possibility of...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...
Showing: 181 - 200 of 575 results