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Showing: 1 - 12 of 12 results

  1. Plan Your Experiment

    Type
    Guide
    ... Many different activators exist, including the multi-plasmid SAM system. Select Expression System To ...

  2. Molecular Biology Reference

    Type
    Guide
    ...Sequencing (NGS) comes in. NGS is a high-throughput, multi parallel sequencing platform that can generate sequencing...combinations each amino acid is encoded for by multiple codons. Plasmids and Recombinant DNA Technology... as cloned DNA, and this process of generating multiple identical copies of a recombinant DNA molecule...the bacterial host. Each bacterium can contain multiple copies of an individual plasmid, and ideally would...bacteria by bacterial transcriptional machinery. Multiple Cloning Site (MCS) Short segment of DNA which ...pressure to maintain and replicate your plasmid over multiple generations. More information relating to antibiotic...puzzle pieces in only a few reactions.There are multiple approaches to acquire NGS but one of the most ...

  3. Antibody Guide

    Type
    Guide
    ...five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers. ...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...allows for the amplification of signals by having multiple secondary antibodies binding to a primary antibody..., which is typically the number of antibodies multiplied by two, as each antibody has two binding sites...

  4. CRISPR Guide

    Type
    Guide
    ...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...

  5. Adenovirus Guide

    Type
    Guide
    ...genomes range between 26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted...harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes. The pAdTrack...that the infection rates are equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy... also been developed as vaccine candidates for multiple infectious diseases and prostate cancer. Cancer... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...integration sites have led to the development of multiple genome-wide analysis pipelines to assess viral...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging.... (2021). CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral...Mandel, R. J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ... unique gene targeting sequence, but there are multiple sequences targeting each gene in the overall library...lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen can help...library screens, cells are infected at a very low multiplicity of infection (MOI) to increase the odds that...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Hybrid capsids are engineered capsids derived from multiple different serotypes, which can further alter transduction... the CNS. Concatemer A DNA segment composed of multiple, repeated DNA sequences linked end-to-end. Episome... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  9. Cloning

    Type
    Guide
    ... same sites in the same orientation within its multiple cloning site . Due to their short length, it is...cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen orientation, and...

  10. Optogenetics Guide

    Type
    Guide
    ...activation wavelengths also make it possible to combine multiple opsins in the same experiment. For example, you...196:1-28. PMID 20303157 Han X, Boyden ES. 2007 Multiple-color optical activation, silencing, and desynchronization...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  12. Chemogenetics Guide

    Type
    Guide
    ... et al. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron...
Showing: 1 - 12 of 12 results