We narrowed to 14 results for: multi

...assembly of single and multi-gene constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) ...of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR editing, and more. Fungal ...vectors containing several cassettes and generate multi-construct transgenic plants. GreenGate 2.0 Toolkit...Cloning System (Lohmann) to create plasmids with multi-gene constructs for plant transformation. GoldenBraid...Irina Borodina Plasmids for assembly of single and multi-gene constructs for integration into Y. lipolytica...containing 96 parts and acceptor vectors for generating multi-part assemblies in either integrative or self-replicating...Michael Springer 160 plasmids to generate single and multi-gene constructs for Xanthobacter or Roseixanthobacter...

...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your...while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs...

...sequencing”) comes in. NGS is a high-throughput, multi-parallel sequencing platform that can generate sequencing...combinations and only 20 natural amino acids, so multiple codons can encode the same amino acid — a phenomenon...providing a survival advantage to the bacterial host. Multiple Cloning Site (MCS) A short segment of DNA which... as cloned DNA, and this process of generating multiple identical copies of a recombinant DNA molecule...for Plasmid Selection Each bacterium can contain multiple copies of an individual plasmid, and ideally would...pressure to maintain and replicate your plasmid over multiple generations. It is important to distinguish that...puzzle pieces in only a few reactions. There are multiple approaches to acquire NGS, but one of the most...

...five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers. ...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...allows for the amplification of signals by having multiple secondary antibodies binding to a primary antibody..., which is typically the number of antibodies multiplied by two, as each antibody has two binding sites...

...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...

...genomes range between 26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted...harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes. The pAdTrack...that the infection rates are equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy... also been developed as vaccine candidates for multiple infectious diseases and prostate cancer. Cancer... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

...integration sites have led to the development of multiple genome-wide analysis pipelines to assess viral...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging.... (2021). CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral...Mandel, R. J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

...about CRISPR tools in our CRISPR Guide . There are multiple types of CRISPR libraries: Knockout : CRISPR knockout...and noncoding RNA studies. Each library contains multiple shRNA sequences for each target gene – a true ...screen should show consistent results from the multiple shRNAs that target it. shRNA libraries may also... screens, cells are transduced at a very low multiplicity of infection (MOI) to increase the odds that...

...Hybrid capsids are engineered capsids derived from multiple different serotypes, which can further alter transduction... the CNS. Concatemer A DNA segment composed of multiple, repeated DNA sequences linked end-to-end. Episome... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

...popular restriction enzyme sites, usually within the multiple cloning site (MCS). You can easily move (subclone... cloning is that it allows for the assembly of multiple fragments of DNA in the chosen orientation at ...

...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

...activation wavelengths also make it possible to combine multiple opsins in the same experiment. For example, you...PMID: 20303157 Han, X., & Boyden, E. S. (2007). Multiple-color optical activation, silencing, and desynchronization...

...transcription elongation factor promoter TRE Inducible Multiple Tetracycline response element promoter; often ...

..., B. L. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron...