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We narrowed to 304 results for: cas9

Showing: 201 - 220 of 304 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ... samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated them...genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all cases, they did not observe...

  2. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy...are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in ...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify the worm genome...be useful to our model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour...

  4. The Twelve Days of CRISPR

    Type
    Blog Post
    ...to me: methods for adenoviral delivery of CRISPR/Cas9.  On the twelfth day of CRISPR, Addgene gave to ...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ... codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the...Applications (CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact that ...CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

  10. Plan Your Experiment

    Type
    Guide
    ...). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the design...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...site. Consider alternative Cas9 enzymes with different PAM sequences or Cas9 enzymes with flexible PAM .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with different base editing ...We will focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these...

  12. Call for Guest Bloggers

    Type
    Blog Post
    ...editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein...

  13. CRISPR Cheat Sheet

    Type
    Blog Post
    ...the gRNAs to cut particular DNA or RNA sequences. Cas9 is a very commonly used CRISPR nuclease. Note:...versions of CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease...

  14. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...1st time CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About... Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest...co-expressed, the gRNA targets the dCas9 to the loci of interest and dCas9 is biotinylated by BirA. From here...To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ... cell line that co-expresses three components: dCas9 with an added biotin acceptor site BirA, a biotin...be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride throughout all of these ... CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally, CAPTURE 3C-based...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility...

  17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) ...Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance...Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR ...High-Efficiency Genome Editing in Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications...
Showing: 201 - 220 of 304 results