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We narrowed to 3 results for: cas9

Showing: 1 - 3 of 3 results
  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...expansion of individual A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast...inferred by the differences in colony size. Figure 2: Cas9 expression in monoclonal cell lines generated from...additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts...monoclonal, 1-5). Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is ...
  2. Video Library

    Type
    Protocol
    ... deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol Over-Agar...
Showing: 1 - 3 of 3 results