Isolating a Monoclonal Cell Population by Limiting Dilution
Type
Protocol
...expansion of individual A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast...inferred by the differences in colony size. Figure 2: Cas9 expression in monoclonal cell lines generated from...additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts...monoclonal, 1-5). Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is ...