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Browse Addgene's collection of video content. Find video protocols, tips on using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols page. Please feel free to email us at [email protected] with any questions.

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Protocols

Video Link Description Related Page
Making LB Agar Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol
Streaking Bacteria on Plates Isolate single bacterial colonies on an agar plate Streaking Bacteria on Plates Protocol
Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction Digest Protocol
Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions for Storing and Handling Plasmids
Genomic Deletions with CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol
Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic Plating Protocol
AAV Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol
Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple tips
Bacterial Transformations We walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency Bacterial Transformation Protocol
Inoculating a Liquid Bacterial Culture How to prepare and grow bacteria in a liquid medium Inoculating a Liquid Bacterial Culture Protocol
Getting Started with Tissue Culture Tips and tricks for working with tissue culture in the lab. Blog post: 10 Basic tips for mammalian cell culture
Aseptic Technique Aseptic technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility while working.
AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol
Agarose Gel Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification. Agarose Gel Electrophoresis Protocol
Purifying DNA from an Agarose Gel Gel purification allows you to isolate and purify DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an Agarose Gel Protocol


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How-To Videos

Video Link Description Related Page
MTA Guide - Requests Your guide to the MTA process when requesting samples from Addgene Technology Transfer
How to Order A step-by-step walkthrough of Addgene's ordering process Ordering Page
MTA FAQs Answers to frequently asked questions about the MTA process Help Center
How to Deposit Your guide to depositing plasmids with Addgene Depositing Page


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Career Videos

Video Link Description
Jessica Welch, PhD In this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica Welch to discuss her career path, differences in research culture between the US and Australia, and advice for current graduate students considering their future careers.
Eric J. Perkins, PhD In this installment, we sit down with Senior Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific community.
Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current projects in the works, and all of the things she loves about working at Addgene!
Maria Soriano Maria Soriano, from Addgene's UK office, discusses her career in science, the importance of international collaboration, and offers career advice for scientists looking to expand their international reach.
Joel McDade Joel McDade, Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice for grauated students pursuing non-academic careers.