We narrowed to 298 results for: IRES

... were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide sequences...

...conditional knockout. This experimental design requires two rounds of recombination – the first removes...

...Additionally, in vitro assembly of NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological...

...end-joining)? MMEJ, a form of alternative end-joining, requires only very small homology regions (5-25 bp) for...

...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

...of - and ExM is no exception. First, expansion requires fixed samples. This limitation may be the both...

...expressed in bacteria, which means their production requires animal sacrifice and precludes genetic engineering...

...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...

...PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds...

...domain. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and...

...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...

...strand is synthesized (Figure 1). Sanger sequencing requires four separate reactions, where a fraction of one...

...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...

...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...

...passages. Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing...