We narrowed to 298 results for: IRES

1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ... were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide sequences...

2. Plasmids 101: Knockout/Knock-In Plasmids

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   Blog Post

   ...conditional knockout. This experimental design requires two rounds of recombination – the first removes...

3. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...Additionally, in vitro assembly of NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological...

4. PhD Applications After COVID

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   Blog Post

   ...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...

5. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ...end-joining)? MMEJ, a form of alternative end-joining, requires only very small homology regions (5-25 bp) for...

6. Which Fluorescent Protein Should I Use?

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   Blog Post

   ...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

7. An Introduction to Adenovirus

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   Blog Post

   ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

8. Getting to Know Expansion Microscopy

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   Blog Post

   ...of - and ExM is no exception. First, expansion requires fixed samples. This limitation may be the both...

9. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...expressed in bacteria, which means their production requires animal sacrifice and precludes genetic engineering...

10. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...

12. Plasmids 101: Colony PCR

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    Blog Post

    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...

13. CRISPR Antimicrobials

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    Blog Post

    ...PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds...

14. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...domain. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and...

15. Plasmids 101: Protein tags

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    Blog Post

    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...

17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...initiation. In mammalian cells, robust repression requires targeting dCas9 along with transcriptional repressors...

18. QC Sequencing Technologies at Addgene

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    Blog Post

    ...strand is synthesized (Figure 1). Sanger sequencing requires four separate reactions, where a fraction of one...

19. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...helps increase cellular uptake, but this approach requires compatible cell lines that express viral machinery...

20. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...