We narrowed to 304 results for: pET
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TypeBlog Post...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...
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Antibodies 101: Immunoprecipitation
TypeBlog Post...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered... -
Hot Plasmids - August 2020
TypeBlog Post...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive... -
Lab to Office Culture Shock
TypeBlog Post...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... assortment toxins to claim territory or kill competing microorganisms, but did you know bacteria also... -
Plasmids 101: Gateway Cloning
TypeBlog Post... are performed, the next step is to transform competent E. coli cells and select the positive clones. ... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...But, as so often happens, one gets better with repetition. I learned to look at the event from the company... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post...linear region of the assay. This may be due to competition for binding sites between the primers and the... -
Plasmids 101: Codon usage bias
TypeBlog Post... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...Tetracycline RecA-deficient; best for plasmids with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal ... -
CRISPR Guide
TypeGuide...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...the production of lentiviral vectors that are competent for one round of infection (AKA transduction in... -
Tips for Getting a Faculty Position
TypeBlog Post...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...