We narrowed to 303 results for: pET

1. Transferable Skills Guide: External Collaborations

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   Blog Post

   ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...

2. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...

3. Lab to Office Culture Shock

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   Blog Post

   ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

4. CRISPR-mediated Plant Base Editors

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   Blog Post

   ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...

5. Plasmids 101: Gateway Cloning

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   Blog Post

   ... are performed, the next step is to transform competent E. coli cells and select the positive clones. ...

6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

7. Hot Plasmids - August 2020

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   Blog Post

   ...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...

8. Transferable Skills Guide: Creativity

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   Blog Post

   ...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...

9. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies...

10. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ...number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA...

11. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...linear region of the assay. This may be due to competition for binding sites between the primers and the...

12. Viral Vectors 101: Pseudotyping

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    Blog Post

    ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

13. Getting the Most from Your Lentiviral Transduction

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    Blog Post

    ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...

14. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal ...

15. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...of time and effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger...

16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

    ... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...

17. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...

19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

20. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...the production of lentiviral vectors that are competent for one round of infection (AKA transduction in...