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GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Addgene's Transfection-Ready DNA delivers high-purity, NGS-verified plasmid DNA ready to pipette—no prep required. Available for 27,000+ plasmids.

This post highlights some of the tools Addgene makes available to industry scientists.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

Highlights of CRISPR impact in the drug discovery pipeline prior to clinical advancement.

Addgene welcomes three new members to the Board of DIrectors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe.

Learn all about a highly processive reverse transcriptase, a new nanobody toolkit, and CRISPR-based tools for monitoring protein-DNA interactions

Learn how you can use kits from the Arabidopsis Biological Resource Center (ABRC) to bring arabidopsis into the classroom.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

Check out some great blog posts from Addgene Depositors.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Learn all about BosLab, a DIY community science space in Somerville MA.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Interested in how Addgene creates our content, like blog posts and guides? We explain here!

Learn about new plasmids at Addgene including CRISPRa in bacteria, voltage indicators, CRISPR knockout library for mice, and lipoprotein tools.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

A general overview of how and when to use screens vs. selections and the ins and outs of each method.