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We narrowed to 449 results for: crispr

Showing: 241 - 260 of 449 results

  1. CRISPR Plasmids and Resources

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    Collection
    ...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... information you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids...

  2. Site Directed Mutagenesis by PCR

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    Blog Post
    ...technique is also highly relevant in this age of CRISPR; site-directed mutagenesis generally applies to...DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed...a similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...More about Plasmids in Our Plasmids 101 Series Use CRISPR to Generate Specific Genomic Mutations Resources...

  3. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming for...Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing success...

  4. Fueled by Coffee at #SfN14

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    Blog Post
    ... infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited...

  5. CRISPR History and Development for Genome Engineering

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...

  6. Screening for Successful Genome Editing with Digital PCR

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    Blog Post
    ...rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation sequencing technologies...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology... crispr...

  7. CRISPR References and Information

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    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...

  8. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...18,343 Human UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...

  9. CRISPR Guide

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    ...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology.... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide...

  10. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ... you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  11. Microbes: Look, Listen, and Tell

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    Blog Post
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  12. Bikard Lab - CRISPR Repression Collection

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    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur...a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently... DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either...

  13. Worm Expression Resources

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    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...

  14. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids... Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and... forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established...

  15. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound to...mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found in bacteria...

  16. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...

  17. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation... conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... with a genomic region of interest in vivo. For CRISPR-based purification, design your gRNA sequence to...

  18. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...

  19. Zhang Lab CRISPR Page

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  20. CRISPR Plasmids - RNA Editing

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type... CRISPR RNA Editing CRISPR Plasmids...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing in mammalian systems. ...
Showing: 241 - 260 of 449 results