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Showing: 1 - 20 of 95 results
  1. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... Nucleic Acids Res pCRISPR::rpsL CRISPR::rpsL CRISPR ; E.coli Marraffini RNA-guided editing of bacterial...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...rerio) CRISPR ; zebrafish expression Joung Efficient genome editing in zebrafish using a CRISPR-Cas system...
  2. CRISPR Plasmids - Bacteria

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...Synthetic) Huang Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein. Nature. 2017 Jun 15;... editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ...Monoavidin in Mammalian Cells. CRISPR J. 2018 Dec;1:414-430. doi: 10.1089/crispr.2018.0045. SpCas9-NLS 17 amino...
  3. CRISPR Guide

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    ...started using CRISPR in your own research. Generating a Knockout Using CRISPR You can use CRISPR to generate...lentiviral CRISPR libraries? Pooled lentiviral CRISPR libraries (often referred to as CRISPR libraries).... Browse Plasmids: Anti-CRISPR Plan Your CRISPR Experiment Get Started CRISPR is a powerful system that...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (2nd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...Guide CRISPR Guide Jump To: CRISPR Guide Overview Knockout Generation Nickase HDR Base Edit Activation ...Publications CRISPR Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...
  4. CRISPR History and Development for Genome Engineering

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...
  5. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...pInducer-20 Lenti-multi-CRISPR Puromycin Yan An easy and efficient inducible CRISPR/Cas9 platform with improved...expression Lenti-CRISPR pAAV-RSV-SpCas9 pRSV (Other) pRSV Lei The CRISPR/Cas9-created MDM2 T309G...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading...
  6. CRISPR Plasmids - Plants

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...Arabidopsis using CRISPR/Cas9 pUB-Cas9 pTX168 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System....1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3 pG3H-U3Ub CRISPR/Cas9 (Synthetic) Chen A Novel...10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3 pG3B-U3Ub CRISPR/Cas9 (Synthetic) Chen A Novel...10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3 pG3B-U6SC CRISPR/Cas9 (Synthetic) Chen A Novel...
  7. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Human CRISPR Knockout Library 92352 Knockout Human Sabatini and Lander 3rd 50 6,661 Human CRISPR Knockout...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...(mVAL) CRISPR Library 159391 Knockout Mouse Moffat 3rd 4 182 Neelamegham Human GlycoGene CRISPR Library...
  8. CRISPR Plasmids - Activate Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...
  9. CRISPR Plasmids - Repress Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...) pBAD Matsumura CRISPR interference and CRISPR single guide RNA (unpublished) CRISPR interference; works... CRISPR Interfere CRISPR Plasmids: Interfere...Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. CRISPR J. 2020 Oct;3(5):398...Deletion and Integration. CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013. Expression of mTN3...
  10. CRISPR Plasmids - Yeast

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...TEF1p, RPR1p LEU2 Zhao Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption...TEF1p, RPR1p URA3 Zhao Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption...Deletion and Integration. CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013. Expression of mTN3.... K. marxianus CRISPR Plasmid for XYL2 editing pJSK316-GPD pIW601-KmCRISPR Codon optimized...
  11. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...aaaa lentiCRISPRv2 CARPID BASU-dCasRx BASU-dCasRx (Homo sapiens) Yan CRISPR-assisted detection...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...LwCas13a-msfGFP (Homo sapiens) EF1a Zhang RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049...dLwCas13a-eGFP-ZF-KRAB (Homo sapiens) CAG Zhang RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049...
  12. CRISPR Plasmids - Zebrafish

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Danio rerio) CRISPR SP6 Giraldez CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...Cas9 CRISPR ; zebrafish expression CMV Joung Efficient genome editing in zebrafish using a CRISPR-Cas ...Flag CRISPR ; zebrafish expression CMV Joung Efficient genome editing in zebrafish using a CRISPR-Cas ...Expression, CRISPR T3 Chen Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease...
  13. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick dCas9-FokI...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive...
  14. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...pInducer-20 Lenti-multi-CRISPR Puromycin Yan An easy and efficient inducible CRISPR/Cas9 platform with improved...expression Lenti-CRISPR pAAV-RSV-SpCas9 pRSV (Other) pRSV Lei The CRISPR/Cas9-created MDM2 T309G...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading...
  15. CRISPR References and Information

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    ...Information You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration... CRISPR References and Information CRISPR References and Information...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab...Mammalian Cell Lines CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This new open...gRNA Design Green Listed - a CRISPR Screen Tool Design gRNAs for custom CRISPR screens targeting a long or...Cas-OFFinder and Microhomology predictor. CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation... Zhang lab: Tips for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated...
  16. CRISPR Plasmids and Resources

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    ...resources on how to start using CRISPR in your lab. Addgene CRISPR Resources CRISPR Guide : Essential background...information on CRISPR and the basics for planning your first CRISPR experiment. CRISPR 101 eBook : Based...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources ...
  17. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick dCas9-FokI Transcriptional Regulation...). Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available for expression...Puromycin Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid ...nickase Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized...
  18. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...P2A-CASANOVA Niopek Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Oct...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...state. Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...Homo sapiens) CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from...
  19. Zhang Lab CRISPR Page

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol (637.8 KB) CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...
Showing: 1 - 20 of 95 results