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Showing: 1 - 20 of 89 results
  1. CRISPR History and Development for Genome Engineering

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...
  2. CRISPR Guide

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    ...started using CRISPR in your own research. Generating a Knockout Using CRISPR You can use CRISPR to generate...lentiviral CRISPR libraries? Pooled lentiviral CRISPR libraries (often referred to as CRISPR libraries).... Browse Plasmids: Anti-CRISPR Plan Your CRISPR Experiment Get Started CRISPR is a powerful system that...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (2nd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...Guide CRISPR Guide Jump To: CRISPR Guide Overview Knockout Generation Nickase HDR Base Edit Activation ...Publications CRISPR Overview Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...
  3. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...Transcriptional Regulators CRISPR Knockout Library 138015 Knockout Mouse Pu N/A 6 14,671 Mouse CRISPR Knockout Library...18,343 Human UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195...
  4. CRISPR Plasmids and Resources

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    ...resources on how to start using CRISPR in your lab. Addgene CRISPR Resources CRISPR Guide : Essential background...information on CRISPR and the basics for planning your first CRISPR experiment. CRISPR 101 eBook : Based...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources ...
  5. CRISPR References and Information

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    ...Information You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration... CRISPR References and Information CRISPR References and Information...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab...Mammalian Cell Lines CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This new open...gRNA Design Green Listed - a CRISPR Screen Tool Design gRNAs for custom CRISPR screens targeting a long or...Cas-OFFinder and Microhomology predictor. CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation... Zhang lab: Tips for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated...
  6. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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    ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas ...variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases... Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression ...information about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users are also...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...
  7. Plant Plasmids and Resources

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    ... Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana - Tetsuya Higashiyama. A CRISPR/Cas9 ...constructs for CRISPR genome editing in dicotyledonous plants - Johannes Stuttmann. MoClo CRISPR/Cas Toolkit...to Bacteria: CRISPR gRNA Multiplexing Using tRNAs Engineering the Plant Genome Using CRISPR/Cas9 Arabidopsis...Plasmids and Resources You may also like... Plant CRISPR resources Plant SynBio resources Fluorescent Protein...Protein Guide Addgene Blog: Plant Biology CRISPR Cloning ORFeomes RNAi Empty Backbones Fluorescent Proteins...collections for Plant Biology. Check out the Plant CRISPR and Plant SynBio collections! The tables below ...
  8. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids... Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and... forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established...
  9. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation... conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... with a genomic region of interest in vivo. For CRISPR-based purification, design your gRNA sequence to...
  10. Worm Expression Resources

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    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...endogenous tagging. Some examples of useful worm CRISPR resources, along with links to protocols, can be...
  11. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...
  12. Calarco Lab CRISPR Plasmids

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    ...Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design... CRISPR Calarco Lab CRISPR Plasmids Calarco Lab...Design Tools CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in C. elegans . The...CRISPR Cas9 gRNA plasmids from Dr. John Calarco's lab. CRISPR...Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, ...
  13. CRISPR Plasmids - dCas9-FokI

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...
  14. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...state. Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...
  15. Zhang Lab CRISPR Page

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...
  16. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive...
  17. CRISPR-Cas/RGN expression plasmids for human cells

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    ... CRISPR-Cas/RGN expression plasmids for human cells You may also like... CRISPR Guide CRISPR Protocols... CRISPR Joung Lab CRISPR Plasmids Human CRISPR-Cas...gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas/RNA-Guided ...Joung lab CRISPR-Cas/RGN expression plasmids for human cells CRISPR...zifit.partners.org/ZiFiT/ChoiceMenu.aspx by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link ...
  18. CRISPR-Cas/RGN expression plasmids for Zebrafish

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    ...Zebrafish CRISPR-Cas/RGN expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols... CRISPR Joung Lab CRISPR Plasmids Zebrafish...gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas/RNA-Guided ...Joung lab CRISPR-Cas/RGN expression plasmids for Zebrafish CRISPR...zifit.partners.org/ZiFiT/ChoiceMenu.aspx by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link ...
  19. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets...containing gRNA sequences designed to be used with any CRISPR nuclease and function. Please see the publication...
Showing: 1 - 20 of 89 results