We narrowed to 304 results for: cas9

1. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   ...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies...

2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

3. Cloning Mammalian Cells with the Agarose Method

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   ...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...

4. Adenoviral Vector Production and Troubleshooting

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   ...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ...

5. Pooled Library Amplifications

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   ...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

6. Uncovering Ancestral Bias in CRISPR Research

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   ... the guide from binding to the genome or prevent Cas9 from cutting, both of which can introduce significant...

7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

8. Lentiviral Vector Uses and Overview

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   ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

9. CRISPR 101: RNA Editing with Cas13

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   ...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given...

10. Hot Plasmids - November 2023

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    ...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

11. Tips for arabidopsis transformation

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    ... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

12. Rett Syndrome

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    ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...

13. The Fluorescent Vegetables in Aptamer Soup

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    ...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...

16. Technologies Enabled by NanoLuc® Luciferase

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    ...endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous...

17. Bacterial Expression Systems

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    ...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...

18. Tetracycline Inducible Expression

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    ...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa...

19. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

20. Quick Guide to All Things Lentivirus

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    ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...