...versions of non-modular proteins like Cre. Could Cas9-DOG be developed soon to allow genome modification...

...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...

...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies...

..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic reader...

...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...

...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ...

...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

... the guide from binding to the genome or prevent Cas9 from cutting, both of which can introduce significant...

... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given...

...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

...require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... Sabatini and Lander 3rd 50 6,661 Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722...

...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli...

...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

...endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous...

...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...