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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 575 results for: multi

Showing: 321 - 340 of 575 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble...

  5. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...every communication that gets sent out through multiple channels is not reasonable or sustainable. That...will be gray areas that either a) overlap with multiple teams or b) fall into a gap between teams that...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...be packaged in AAV vectors, potentially with multiplexed targets.   Figure 4: CHARM uses a fusion...  Multiple Authors...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ...

  10. Five Popular Model Organisms

    Type
    Blog Post
    ...organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish...making it easy to conduct large experiments over multiple generations. Similarly to other model organisms...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce...

  14. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ...and reprobed with different antibodies allowing multiple targets to be assessed from a single western.  ...used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the primary ...

  15. CRISPR 101: Off-Target Effects

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    Blog Post
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

  17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different... AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...a Group Leader position, what will you need? Multiple first or corresponding author publications (published...interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    ...you can see, AAVs of each serotype can transduce multiple tissues. So how do you choose the right serotype...thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer....

  20. Antibodies 101: Validation

    Type
    Blog Post
    ...antibody works! Multiple (Independent) Antibodies Strategies These strategies use multiple, unique antibodies...
Showing: 321 - 340 of 575 results