We narrowed to 97 results for: dcas9

...and nickase, but we are now adopting dCas9, dCas9-VP64, and dCas9-KRAB in our system,” Sakuma said. “These...

...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study...University of Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal... al. “Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nature...

...options include humanized wt Cas9, dCas9 (transcriptional repressor), and dCas9-VP64 (transcriptional activator...D10A nickase mutant (Cas9n), dCas9 (transcriptional repression), and Fok1-dCas9 (dimeric nuclease). Gateway...then digested using BsmBI and ligated into Cas9 or dCas9-containing destination vectors. Destination vector...allows you to assemble type II-A CRISPR arrays for dCas9-based transcriptional repression. The CRISPathBrick...Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in...

...propagation and keeping that dCas9 sequence in the pool. According to this logic, ω-dCas9 variants recognizing... phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This...NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it would induce transcription...

...experiments, such as transcription factors tethered to dCas9, require long time scales with constitutively expressed...proteins that are not inducible. To further develop dCas9 for synthetic looping, the Phillips-Cremins lab ...-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN...

...creating a new dCas9-VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into ... is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...methylation by fusing catalytically inactive Cas9 (dCas9) with the catalytic domain from DNA methyltransferase... activator developed by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target...target genes (20-40x greater than standard dCas9-VP64 activators) when introduced into mammalian cells....activate target genes, the relatively large size of dCas9-VPR limits one’s ability to package it into lentivirus...transfection precluding the need for virus. Furthermore, dCAS9-VPR expression is under the control of the TRE promoter...

...? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on...

...dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also...

...capability, and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains such...new cell lines or transgenic animals expressing dCas9 as a means of modulating gene expression (6). Altering... dcas9...

...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)... used this technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted...

...strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription and destroy ...system available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription...model. If these escape mutations are frequent, the dCas9-mediated “shock and kill” strategy may be a better...

...Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding...protein-bEFP fusion. Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the...

...TurboCas provides the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, ...proximity ligation tool (Cenik et al., 2024). While dCas9 has been used in combination with proximity labeling...background labeling. By fusing miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created ...

... a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...

...🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in...

... editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein can ...Jacobs-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance...

...antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader...promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi is stably...

...versions of CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease...

... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes,...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A ...56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes...non-targeting controls. This backbone does not contain dCas9. 57,050 unique sgRNAs targeting 18,901 human genes...non-targeting controls. This backbone does not contain dCas9. 57,011 unique sgRNAs targeting 18,899 human genes...Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a ...2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID...