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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: dcas9

Showing: 1 - 3 of 3 results

  1. CRISPR Guide

    Type
    Guide
    ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...imaging of proteins in living cells (Figure 9B) dCas9-VPR - dCas9 fused to several different activation domains...utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin... defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription...target gene CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription...one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A...

  2. Plan Your Experiment

    Type
    Guide
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ...edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with different base editing...be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene... the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need...N. J. (2023). Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent...

  3. Modular Cloning Guide

    Type
    Guide
    ...up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems...
Showing: 1 - 3 of 3 results