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We narrowed to 303 results for: pET

Showing: 21 - 40 of 303 results

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...operator. Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...agent (0.4 mM IPTG is a good start if you’re using a pET vector) and antibiotic for plasmid selection. Remove... for protein overexpression in E. coli, such as pET30a. If working with DNA that is refractory to enzymatic...your resultant plasmid libraries into chemically competent E. coli and analyze transformants for appropriately...order to reduce the chances of some clones being outcompeted by others in liquid culture, dilute and plate...transformation should be performed using a highly competent strain that you can use for long-term library ...probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of nitrocellulose membrane and...inhibitor cocktail. Line the bottom of 20 empty Petri dishes with thick filter paper and saturate each...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...available! Interested in purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for...generated mix of fluorescent proteins, determined by competing recombination events at the genetic level. Upon...

  9. Genetic Code Expansion

    Type
    Collection
    ...jannaschii p-azido-l-phenylalanine Bacterial TAG Peter Schultz 31190 pEVOL-pBpF p-benzoylphenylalanine ...jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter Schultz 48215 pULTRA-CNF tyrosyl-tRNA synthetase... para-cyanophenylalanine (pCNPhe) Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli 3-(6-acetylnaphthalen...ylamino)-2-amino-propanoic acid (Anap) Mammalian TAG Peter Schultz 49086 pDULE-ABK PylRS M. barkeri aliphatic...aliphatic diazirine amino acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA...coli various unnatural amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri... variety of unnatural amino acids Mammalian TAG Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine...

  10. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine...26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL ...

  11. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

  12. CRISPR Guide

    Type
    Collection
    ...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ...

  13. Fluorescent Proteins: FRET

    Type
    Collection
    ...mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530 104,000 0.77 5.3 2.3 CYPet-N1 , YPet-N1 mTFP1 mVenus 462 ...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ... luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector... investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector... investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion...

  16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... These are sandwich, competitive, direct, and indirect. Sandwich and competitive ELISA use antibodies ...et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also used ... be adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...epitopes on the target protein, so they do not compete with each other. This antibody is also conjugated...to your protein of interest) will bind to the competing antigen in the wells while the protein-antigen...protein of interest in the original sample. The competitive ELISA is best used for detecting antigens that...sandwich ELISA. While the most common type of competitive ELISA is a modification of the sandwich ELISA...

  17. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antibody can be used. In a competitive ELISA a multiwell plate is coated with a competing antigen (green).  The...antibodies are used.  Competitive ELISAs Scientists typically use a competitive or inhibition ELISA when... of ELISA are direct, indirect, sandwich and competitive. In a direct ELISA a reporter enzyme conjugated...sample, the less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA... level of antigen in the sample whereas in a competitive ELISA the reporter signal is inversely proportional...sandwich ELISA. In this method, the target antigen competes with a reference antigen for binding to a primary... direct, indirect, and sandwich ELISAs, in a competitive ELISA the signal intensity is inversely proportional...

  18. Science as Art

    Type
    Blog Post
    ...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this... University’s Annual Art in Science competition. This competition has gained so much public interest for...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old international...been featured in Time magazine . Other smaller competitions are now taking place and gathering momentum,...international competition that last year received over 2,000 entries. Do you mix your passion for art and...

  19. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused...Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined...Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   Two methods... with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    The team showed...strains and environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown...possible with Mobile-CRISPRi. As a proof of principle, Peters and colleagues constructed a 40-member library ...microbiology 4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of...PMID: 30617347. PubMed Central PMCID: PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society...
Showing: 21 - 40 of 303 results