We narrowed to 1,047 results for: sta

... included a function section for if you want to stably express an insert using viral vectors, are studying...attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones on our Plant...proteins are excellent tools for further understanding the function of your favorite gene. For example...the stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing...experiments, scientists often want to create stable cell lines in which the expression cassette of ...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA ...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing...Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin...Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt...community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids...Genetically Engineered Machine Foundation Registry of Standard Biological Parts (Link opens in a new window) ...on Syn Bio SEVA (Link opens in a new window) — Standard European Vector Architecture Open Plant (Link ...

...system that allows sustained cell loss and ablation of previously resistant cell types. CreLite... fish in the minnow family. It has long been established as a powerful vertebrate model organism for the...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons...CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease...Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...

... and regression of speech. In contrast to the sustained loss of skills in neurodegenerative conditions...self-limited, after which a period of developmental stabilization or even limited recovery of skills occurs. Diagnosis...in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular level. They...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease and for the development...resource by contributing your plasmids! To get started with your deposit, please click here ....

...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-...expressed and purified as described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel...J Am Chem Soc. 126(29):8896-7. PMID:15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. ...

...Lentiviral Stable RNA < 8 kb 80–130 nm Dividing/Non-dividing Yes Low Gamma-retroviral Stable RNA < 8 kb...non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently integrated ...expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide...80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb 18–26 nm Dividing...

...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory... and resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids for... Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus...synbio experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids...

...( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut...codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang...transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A,...lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE...fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4)...

...acceptor fluorophore if they are within a certain distance. You can test if two proteins bind or interact...mammalian and bacterial FRET-related vectors and standards. The Hoschschild Bacterial Two-Hybrid (B2H) System...study the effects of protein expression in the stationary phase. In these cases, you may want to control...pTetO Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia...68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose... (mCherry) Mycobacterium sp. Tanya Parish 24657 pASTA3 Promoter activity Fluorescence (tdTomato) Mycobacterium...

...molecular reagents for biology have propelled our understanding of neural circuit functionality. Precisely expressing... ORF that is sandwiched between the sites; the starting direction of these ORF fragments (which have become...experimental design. The Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...Deisseroth K, Callaway EM, Oberhuber M, Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of...

... hope to aid scientists who want to immediately start cloning upon arrival of their plasmid from Addgene...required when one receives plasmids in bacterial stabs. DNA Service Details Amount 2 µg Volume 20 µl Concentration...plasmid page to find current pricing information Standard Quality Control Plasmid verified by next generation...Customer Support Teams are also available to provide assistance at [email protected] . Don’t See What You’re Looking...

...Cas9 orthologs have also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and ...t need to be an expert to see results! Getting Started with CRISPR Addgene has empowered researchers to...DA, Horvath P. 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315...: 27088723 Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ,...Zhang F. 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature . 520(7546):186-91. PMID...

...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the...HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...Oct 19. PubMed . In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott ...

... cell. Scientists can take advantage of this to stably integrate transgenes of interest into a variety...cells. See the Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT...GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV...expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells. Alice...

...continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent...adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc...This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease...can be faster, bypasses intermediate pluripotent stages, and can occur without cell division, all of which...from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult...

...to an antigen, a particular part of a foreign substance. In addition to being useful for our bodies to...fragment of an antibody, which could offer increased stability and ease of production due to their small size...McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas...and secreted proteins suitable for wholemount immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456...

...the incorporation of amino acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs...protein of interest, four key modifications to the standard translation machinery are required: The ncAA is... substrate for endogenous aaRS enzymes to load standard amino acids. tRNA and aaRS pairs from Methanocaldococcus... scale, the expanded genetic code can help us understand and evolve proteins for various purposes from...consider. If you are working off of a previously established protocol, make sure to match the growth medium...for multi-site incorporation of two distinct non-standard amino acids. Derived from C321.deltaA, by replacing...

...By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep...viral vector preparations, we set both minimum standards and typical titers, though actual yields may vary...depend on your location. Share Your Results Once you start getting results from your AAV experiments, remember...answer isn’t in the Help Center or you need more assistance with troubleshooting, we want to hear from you...

...GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate...differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously...can be stuck in a constitutively active form, constantly activating pathways for cell growth and potentially...TIAM TIAM1 TIAM2 T-cell lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous...

...metal ions, scientists have adapted an antibiotic resistance operon from E. coli : the tetracycline repressor...tet-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be made to...promoter, and type of transduction (transient or stable) may depend on the desired sensitivity, level of... lineages and transcriptional and proliferative state of each cell in a population. Proliferation history...