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We narrowed to 115 results for: sta

Showing: 1 - 20 of 115 results

  1. Synthetic Biology - Assembly Standards Guide

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    Collection
    ...Assembly standards define standard prefix and suffix sequences that make it easy to combine components... Assembly Standards Guide SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog...use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix.... BioBrick BioBrick-2 BglBrick Silver Standard Freiburg Standard BioBrick (RFC 10) Prefix: GAATTC GCGGCCGC...visit iGEM: BglBrick – Berkeley Standard (RFC 21) Back to Top Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC...more info, visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC...following table provides information for the assembly standards most commonly used by part registries, including...

  2. Fluorescent Protein Guide: FRET

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    ...interactions or conformation changes, as well as FRET standards....pair, the FRET efficiency strongly depends on the distance between the donor and acceptor molecules and can... can therefore be used to calculate the distances between the donor and acceptor. FRET is often used ...protein-protein interactions and a series of FRET standards . Pre-constructed fluorescent biosensors targeting...between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized... values and were developed to be used as FRET standards to calibrate the numerous and disparate methods...linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent proteins connected...

  3. Antibody Guide

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    ... the constant region, and is shown in dark blue and dark orange. The constant region is a stable part ...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant region...techniques use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent...sequencing, and enables the variable region and constant regions of the antibody to be independently manipulated...Structure Figure 1: Structure of an antibody A standard antibody is made up of four protein chains: two...responsible for producing antibodies, can change the constant region, and therefore change the isotype of antibodies...capable of recognizing and binding epitopes on constant regions of other antibodies. This has useful research...

  4. Immunology Research Plasmids and Resources

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    Collection
    ...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...immunoglobulin heavy constant gamma 2 (G2m marker) DKFZp686I04196 IGHG3 immunoglobulin heavy constant gamma 3 (G3m...immunoglobulin lambda constant 1 (Mcg marker) IGLC IGLC2 immunoglobulin lambda constant 2 (Kern-Oz- marker...immunoglobulin lambda constant 3 (Kern-Oz+ marker) IGLC IGLC6 immunoglobulin lambda constant 6 (Kern+Oz- marker...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...

  5. CRISPR Guide

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    ...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various applications...applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics In this...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy...

  6. Neurodegeneration Plasmid Collection

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    Collection
    ...telangiectasia Michael Kastan 32300 hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C..., FTD Stefan Stamm 203366 Tau BA 12->7 I260V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203367 Tau BA..., FTD Stefan Stamm 203368 Tau BA 12->7 G272V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203371 Tau BA..., FTD Stefan Stamm 203372 Tau BA 12->7 P301S MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203373 Tau BA..., FTD Stefan Stamm 203374 Tau BA 12->7 S305N MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203375 Tau BA..., FTD Stefan Stamm 203376 Tau BA 12->7 K317M MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203378 Tau BA...

  7. AAV for Neuronal Tracing

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    ...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

  8. Plan Your Experiment

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    ...Once you have a clear understanding of your experimental goal, you are ready to start designing the specific...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact...experiment) and the expression time (transient or stable). For plasmid and viral-based approaches, the species...

  9. Trimmer Lab NeuroMab Collection

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    ...Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...Mouse IgG2a 128635 Anti-Ankyrin-G (staining) [N106/43R] Ankyrin-G (staining) Human Mouse IgG2a 128636 Anti-LRRTM4...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR... Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17...Mouse IgG2a 177521 Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2...Mouse IgG2b 206664 Anti-TRIP8b (constant) [N212/17R-2b] TRIP8b (constant) Rat Mouse IgG2b 206665 Anti-SynCAM4...

  10. Antibody Production

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    ...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...alongside serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein...and a standard curve is generated. The final antibody concentration is calculated from the standard curve...by Addgene scientists. They are generated using standard methods individually optimized for each specific...opens in a new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw... antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, freezing at...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However...

  11. University of Florida Serotype Testing Panel for the Eye and Brain

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    ...transduction of the eye and other tissues. As part of our standard viral production , these viral vectors undergo...The following serotypes were developed in Arun Srivastava’s or Shannon Boye’s lab at the University of Florida...derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF) The AAV2(trpYF) serotype shows...The AAV2(trpYF) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV2 capsid and ...AAV6(dbY-F+T-V) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV6 capsid and ... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties...

  12. Viral Production

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    ...electrophoresis (PAGE) followed by silver staining or SYPRO Ruby staining and the molecular weight and relative...after negative staining. Empty vector particles can be identified after negative staining and appear darker...of Viral Production Viruses are generated using standard methods that have been optimized for each specific... 2–3 fold higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania...high quality viral vectors by optimizing and standardizing production protocols and performing rigorous...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically...

  13. Luciferase Plasmid Collection

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    ...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which... signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular...luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments, but is too dim... luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720...luciferases with altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use...protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory Region)...reporter. These plasmids improve on the original STARR-seq by using the bacterial ORI as the core promoter...

  14. Fluorescent Protein Guide: Biosensors

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    ... cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded ...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025...FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ...and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor of the...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...

  15. Adeno-associated virus (AAV) Plasmids

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    ...-iCAP-PHP.eB PHP.eB AAV packaging plasmid (non-standard), expressing Rep2 and PHP.eB Cap controlled by...pUCmini-iCAP-PHP.S PHP.S AAV packaging plasmid (non-standard), expressing Rep2 and PHP.S Cap controlled by ...-iCAP-PHP.V1 PHP.V1 AAV packaging plasmid (non-standard), expressing Rep2 and PHP.V1 Cap controlled by...pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled by...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... included a function section for if you want to stably express an insert using viral vectors, are studying...attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones on our Plant...proteins are excellent tools for further understanding the function of your favorite gene. For example...the stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing...experiments, scientists often want to create stable cell lines in which the expression cassette of ...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA ...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd...

  17. Lentivirus Plasmids

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    ...shRNA empty plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – ...significant immune response. These viruses also integrate stably into the host genome, enabling long term transgene...Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on ...fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven...

  18. Synthetic Biology - Overview

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    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing...Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin...Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt...community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids...Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...Collection - Open access papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant...

  19. Zebrafish Plasmid Collection

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    ...system that allows sustained cell loss and ablation of previously resistant cell types. CreLite... fish in the minnow family. It has long been established as a powerful vertebrate model organism for the...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as Tol2 transposons...CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease...Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...

  20. Rett Syndrome

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    ... and regression of speech. In contrast to the sustained loss of skills in neurodegenerative conditions...self-limited, after which a period of developmental stabilization or even limited recovery of skills occurs. Diagnosis...in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular level. They...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease and for the development...resource by contributing your plasmids! To get started with your deposit, please click here ....
Showing: 1 - 20 of 115 results