Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,046 results for: sta

Showing: 1 - 20 of 1046 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Assembly standards define standard prefix and suffix sequences that make it easy to combine components... Assembly Standards Guide SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog...use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix.... BioBrick BioBrick-2 BglBrick Silver Standard Freiburg Standard BioBrick (RFC 10) Prefix: GAATTC GCGGCCGC...visit iGEM: BglBrick – Berkeley Standard (RFC 21) Back to Top Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC...more info, visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC...following table provides information for the assembly standards most commonly used by part registries, including...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sample.  How do I make the standards for a standard curve? You create a standard curve by serially diluting...use qPCR with a standard curve to titer their viruses; food allergy labs use standard curves with ELISAs...them in their assays.  What is a standard curve? Scientists use standard curves to determine the concentration...diluting a known control sample, called a standard. The ideal standard curve has at least five dilutions, with... measurements. You'll need to prepare the standard, standard dilutions, and unknown sample(s) in the same...or volumes too large for a standard micropipette (> 1,000 µL). The standard dilution series should be ... value. How do I plot and use a standard curve? To create a standard curve, you graph the measured response...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters...keep gene expression stable regardless of copy number, the team made use of stabilized promoters. In contrast...and keeping its expression level constant. In the case of the stabilized promoters from Segall-Shapiro et...gene expression also remained stable regardless of plasmid copy number. Stable gene expression whether growing... were expressed. What’s the use of stable gene expression? Constant gene expression can enable more confidence... to unregulated promoters that are always on, stabilized promoters incorporate feedback loops such as ...with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered against the effects of copy...

  4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...tested at the beginning. Variability source Standardization parameters Animal Strain, age, sex, housing...conditions (individually versus in groups), health status, diet, cage enrichment, stress level, origin (vendor...Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing: High expression...situations such as learning and memory or emotional states, for example anxiety or depression. In upcoming...

  5. Antibody Guide

    Type
    Collection
    ... the constant region, and is shown in dark blue and dark orange. The constant region is a stable part ...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant region...techniques use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent...sequencing, and enables the variable region and constant regions of the antibody to be independently manipulated...Structure Figure 1: Structure of an antibody A standard antibody is made up of four protein chains: two...responsible for producing antibodies, can change the constant region, and therefore change the isotype of antibodies...capable of recognizing and binding epitopes on constant regions of other antibodies. This has useful research...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...immunoglobulin heavy constant gamma 2 (G2m marker) DKFZp686I04196 IGHG3 immunoglobulin heavy constant gamma 3 (G3m...immunoglobulin lambda constant 1 (Mcg marker) IGLC IGLC2 immunoglobulin lambda constant 2 (Kern-Oz- marker...immunoglobulin lambda constant 3 (Kern-Oz+ marker) IGLC IGLC6 immunoglobulin lambda constant 6 (Kern+Oz- marker...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...

  7. CRISPR Guide

    Type
    Collection
    ...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various applications...applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics In this...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...addition of a structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased synergy...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...telangiectasia Michael Kastan 32300 hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C..., FTD Stefan Stamm 203366 Tau BA 12->7 I260V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203367 Tau BA..., FTD Stefan Stamm 203368 Tau BA 12->7 G272V MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203371 Tau BA..., FTD Stefan Stamm 203372 Tau BA 12->7 P301S MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203373 Tau BA..., FTD Stefan Stamm 203374 Tau BA 12->7 S305N MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203375 Tau BA..., FTD Stefan Stamm 203376 Tau BA 12->7 K317M MAPT Flag CMV Parkinson's, FTD Stefan Stamm 203378 Tau BA...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...VenusY67C FRET reference standards mCerulean-mVenus FRET efficiency standards Thaler et al. (2005) Quantitative...Fluorescent Proteins: FRET FRET Pairs FRET Calibration Standards Additional Resources Förster (or Fluorescence)...Because this process is extremely sensitive to the distance between the fluorophores at the single-nanometer...Acceptor quantum yield, R 0 : Förster Radius (nm, distance at which 50% FRET efficiency occurs), J(λ) : Overlap...for more detailed discussion. FRET Calibration Standards The articles or collections listed below feature...positive and negative FRET controls or calibration standards consisting of donor and acceptor proteins fused...varying lengths. Description Article ECFP-EYFP FRET standards, fusion proteins with linkers of varying length...

  11. Antibody Production

    Type
    Collection
    ...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...alongside serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein...and a standard curve is generated. The final antibody concentration is calculated from the standard curve...by Addgene scientists. They are generated using standard methods individually optimized for each specific...opens in a new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw... antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, freezing at...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However...

  12. Plan Your Experiment

    Type
    Collection
    ...Once you have a clear understanding of your experimental goal, you are ready to start designing the specific...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact...experiment) and the expression time (transient or stable). For plasmid and viral-based approaches, the species...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...Mouse IgG2a 128635 Anti-Ankyrin-G (staining) [N106/43R] Ankyrin-G (staining) Human Mouse IgG2a 128636 Anti-LRRTM4...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR... Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17...Mouse IgG2a 177521 Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2...Mouse IgG2b 206664 Anti-TRIP8b (constant) [N212/17R-2b] TRIP8b (constant) Rat Mouse IgG2b 206665 Anti-SynCAM4...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...212020 pcDNA3/mStayGold(c4)=UtrCH Actin filaments Utrophin calponin homology domain mStayGold Atsushi Miyawaki...Miyawaki 212019 pcDNA3/F-tractin=mStayGold Actin filaments F-tractin mStayGold Atsushi Miyawaki 229608 mBaoJin-lifeactin...Petsko 221899 mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo Microtubules alpha-Tubulin mStayGold Janelia Integrative...Gadella 212022 pcDNA3/td5StayGold(c4)=GianCreg Golgi apparatus Giantin td5StayGold Atsushi Miyawaki 231777...Lamp1 RFP Walther Mothes 221902 Lamp1_mStayGold Lysosomes Lamp1 mStayGold Janelia Integrative Imaging 231778...mGreenLantern Gregory Petsko 221898 mito-mStayGold_pTwistCMVBetaGlobWPRENeo Mitochondria Mitochondrial targeting...targeting sequence (COX8) mStayGold Janelia Integrative Imaging 189774 p4xmts-mScarlet3_N1 Mitochondria 4x...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mStayGold (StayGold-E138D) 497 504 126 4.6 14 min Monomer pcDNA3-10His-mStayGold (E138D) - ...dimerization prsTagRFP-N1 - Mammalian Expression prsTagRFP-C1 - Mammalian Expression pBAD/HisD-rsTagRFP - Bacterial...pET-MCN-10His-mStayGold (E138D) - Bacterial Expression pJRA110 - Plant Expression mStayGold 499 510 136 ...136 4.8 Monomer pRSETB/mStayGold - Bacterial Expression mBaoJin (mStayGold) 500 508 119 4.4 7.6 min Monomer...maximal value. Structure describes the oligomeric state of the protein. Many FPs are monomers at low concentration...pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish...Mammalian Expression mGeos-M-C1 - Mammalian Expression rsTagRFP 567 585 440 (Off to On) 567 (On to Off) 4.0 6.6...

  16. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...transduction of the eye and other tissues. As part of our standard viral production , these viral vectors undergo...The following serotypes were developed in Arun Srivastava’s or Shannon Boye’s lab at the University of Florida...derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF) The AAV2(trpYF) serotype shows...The AAV2(trpYF) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV2 capsid and ...AAV6(dbY-F+T-V) serotype was developed by Arun Srivastava’s lab. It is derived from the AAV6 capsid and ... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency... future publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties...

  17. Viral Production

    Type
    Collection
    ...electrophoresis (PAGE) followed by silver staining or SYPRO Ruby staining and the molecular weight and relative...after negative staining. Empty vector particles can be identified after negative staining and appear darker...of Viral Production Viruses are generated using standard methods that have been optimized for each specific... 2–3 fold higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania...high quality viral vectors by optimizing and standardizing production protocols and performing rigorous...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids are typically...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded ...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025... FRET sensors to monitor intracellular Zn 2+ homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten ...and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn 2...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn 2+ Secretion of...sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor of the...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which... signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular...luminescence. Gaussia luciferase is secreted and more stable, enabling time course experiments, but is too dim... luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720...luciferases with altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use...protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory Region)...reporter. These plasmids improve on the original STARR-seq by using the bacterial ORI as the core promoter...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...-iCAP-PHP.eB PHP.eB AAV packaging plasmid (non-standard), expressing Rep2 and PHP.eB Cap controlled by...pUCmini-iCAP-PHP.S PHP.S AAV packaging plasmid (non-standard), expressing Rep2 and PHP.S Cap controlled by ...-iCAP-PHP.V1 PHP.V1 AAV packaging plasmid (non-standard), expressing Rep2 and PHP.V1 Cap controlled by...pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled by...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by...
Showing: 1 - 20 of 1046 results