We narrowed to 139 results for: yeast

...autophagy, Rosella is targeted to the vacuole in yeast or the lysosome in mammalian cells. This decrease... Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3 (2007): 181-206. PubMed... vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed PMID: 18094608...

...normal kit protocols.  Yeast plasmids – If you’re extracting plasmids from yeast, instead of bacteria, ...manufacturers (such as Zymo and TakaraBio) offer yeast-plasmid specific kits.  Medium/High-throughput –...Some kits that may not be explicitly labeled as “yeast”, “transfection-grade”, or “large plasmid” may be...mentioned Endotoxin-free, transfection-grade, BAC, yeast Zymo Beads, column, plate Mini, midi, maxi, giga...columns, plates, buffers 96-well plates Not mentioned Yeast, BAC, transfection-grade While we’ve included...

...antibody production can take place in bacterial, yeast, plant, or mammalian cells. Each of these systems...advantages and disadvantages. Briefly, bacterial and yeast cells are cost-effective and faster-growing than...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system may...of protein you need to produce.  The most common yeast strain used for recombinant antibody production ...

...up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools for mammalian...from the viral service Faster catalysis after yeast-based protein evolution By Joanne Kamens Mateo Sanchez...to optimize another. The authors have created a yeast-based directed evolution approach with some notable...involves selection in the cytosol (instead of on the yeast extracellular surface) which enables tracking of...

...Kinase Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use ...Saccharomyces strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer...

...negative repressible promoter is commonly used in yeast. In the first 24 hours of culture, when glucose ... promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it binds...activity during the late ethanol consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In ...binary system is the GAL4/UAS system isolated from yeast. In this system, UAS basal promoter expression is...applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153...

...Cell surface display systems, such as bacterial, yeast, or mammalian display, allow for the presentation...engineering, and have recently launched a course on yeast display for antibody discovery, to share their expertise... in Tampa, Florida. His projects focus on using yeast display, structural biology, and protein engineering...Kang, B. H., Lax, B. M., & Wittrup, K. D. (2022). Yeast Surface Display for Protein Engineering: library...Ring, A. M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display platform for rapid discovery of ...

... not form undesirable aggregates. Using budding yeast as a model system, Georgieva et al. examined the... Myc tags. Impressively, IT6-GFP did not reduce yeast cell size or viability compared to untagged GFP ...

...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...other disease-linked Arg-mutant proteins both in yeast and human cells in order to determine the specific...

...(nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus...lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, insect expression, ...

...research! This time: Optogenetic repression in yeast Base editing CRISPRi in Caulobacter crescentus Multiplexed... Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit...

...sought to test the suitability of four additional yeast recombinases: KD (from Kluyveromyces drosophilarum...of the mouse brain. Rubin’s work shows that the yeast recombinases KD, B2, B3 and FLP can be used together...

... the surface of a host cell or virus, such as a yeast or bacteriophage species. This approach is useful...Surface display screening can also be conducted with yeast. Created with BioRender.com. Conclusion Have...

...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...demand. The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that...2024). Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with...

...expressed in non-mammalian cells. Yeast expression plasmids The yeast expression constructs contain protein...

... of long-time Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2...What’s more, since the enzymes were ”evolved” in yeast at 30℃, the enzymes function across a much broader...

...Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression... Depositor Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection... . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...proteins in yeast. Depositor...Overexpression Resources This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a ...FPs and tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et...

...selection process is often performed via phage display, yeast display, ribosome display, or mammalian display ... introduced into host cells, such as bacterial, yeast, or mammalian cells, for antibody production and...

...CRISPRa system in yeast is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids...

...tissues; more hardy tissues such as bone, or bacteria/yeast/plant samples will require additional steps to effectively...