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We narrowed to 152 results for: yeast

Showing: 21 - 40 of 152 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...autophagy, Rosella is targeted to the vacuole in yeast or the lysosome in mammalian cells. This decrease... Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3 (2007): 181-206. PubMed... vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed PMID: 18094608...

  2. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...normal kit protocols.  Yeast plasmids – If you’re extracting plasmids from yeast, instead of bacteria, ...manufacturers (such as Zymo and TakaraBio) offer yeast-plasmid specific kits.  Medium/High-throughput –...Some kits that may not be explicitly labeled as “yeast”, “transfection-grade”, or “large plasmid” may be...mentioned Endotoxin-free, transfection-grade, BAC, yeast Zymo Beads, column, plate Mini, midi, maxi, giga...columns, plates, buffers 96-well plates Not mentioned Yeast, BAC, transfection-grade While we’ve included...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ...up here.  Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools for mammalian...from the viral service Faster catalysis after yeast-based protein evolution By Joanne Kamens Mateo Sanchez...to optimize another. The authors have created a yeast-based directed evolution approach with some notable...involves selection in the cytosol (instead of on the yeast extracellular surface) which enables tracking of...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...antibody production can take place in bacterial, yeast, plant, or mammalian cells. Each of these systems...advantages and disadvantages. Briefly, bacterial and yeast cells are cost-effective and faster-growing than...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system may...of protein you need to produce.  The most common yeast strain used for recombinant antibody production ...

  5. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Kinase Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use ...Saccharomyces strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...negative repressible promoter is commonly used in yeast. In the first 24 hours of culture, when glucose ... promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it binds...activity during the late ethanol consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In ...binary system is the GAL4/UAS system isolated from yeast. In this system, UAS basal promoter expression is...applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ... not form undesirable aggregates. Using budding yeast as a model system, Georgieva et al. examined the... Myc tags. Impressively, IT6-GFP did not reduce yeast cell size or viability compared to untagged GFP ...

  9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...(nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus...lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, insect expression, ...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...research! This time: Optogenetic repression in yeast Base editing CRISPRi in Caulobacter crescentus Multiplexed... Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit...

  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...demand. The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that...2024). Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with...

  17. Kit Free RNA Extraction

    Type
    Protocol
    ...tissues; more hardy tissues such as bone, or bacteria/yeast/plant samples will require additional steps to effectively...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...bacteria studies, whereas GFPS65T is better suited for yeast studies (24-30°C). Brightness: Brightness is a measure... to express the FP in acidic environments (e.g. yeast cytosol, which is slightly acidic, or synaptic vesicles...
Showing: 21 - 40 of 152 results