Viral Vectors 101: Calcium Sensors
Type
Blog Post
...the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded...
We narrowed to 30 results for: yfp
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Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or puromycin... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...works. A split FP (e.g., yellow fluorescent protein, YFP) is reunited by the interaction between two PIPs,... -
Tips for arabidopsis transformation
TypeBlog Post...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG)... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...neurons are brightly labeled with GFP (e.g. Thy1-YFP-H mice). However, we recently developed AAV (adeno-associated... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral... -
CRISPR Plasmids - Tagging
TypeCollection...templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent selection... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone to ...A206K) pSYFP2-C1 - Mammalian Expression Hyperfolder YFP (hfYFP) 514 529 72 5.6 21 min Prone to dimerization... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...protein, permeabilized cells Cy5NTAc 43 34-62 YFP Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc... -
Fluorescent Protein Guide: Biosensors
TypeCollection... 2013 Nov 13;7:202. Colin Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in...in physiological range A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability...