We narrowed to 452 results for: crispr
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TypeBlog Post... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...
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How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent... -
Hot Plasmids - May 2022
TypeBlog Post...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the... -
All in a Twist: dsRNA
TypeBlog Post...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13 RNA Interference ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ... -
Synthetic Biology - Overview
TypeCollection...Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post... CRISPaint Gene Tagging Kit SpyTag/SpyCatcher CRISPR-assisted insertion tagging Kit; integration of ... -
Developing Lab Management Software for Biology
TypeBlog Post...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ... -
CRISPR Guide
TypeGuide...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases. Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases... using CRISPR libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...adaptability, CRISPR rapidly became the most popular genome engineering approach. Engineered CRISPR systems ... gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was originally employed to knock out target... -
Plan Your Experiment
TypeGuide... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic... -
Modular Cloning Guide
TypeGuide...multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning (FTK) Fungal Expression, CRISPR Arnold...experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian Expression Takashi...MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering, CRISPR Sylvestre Marillonnet...SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome Engineering, CRISPR, Plant Expression Vladimir...Engineering, CRISPR, Plant Expression Johannes Stuttmann Plasmids for assembly of constructs for CRISPR genome...Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome...(a.k.a. K. phaffii ). NT-CRISPR Plasmid Collection Genome Engineering, CRISPR, Bacterial Expression Anke... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post.... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the...