We narrowed to 444 results for: crispr
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TypeBlog Post...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even...
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Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ... -
Hot Plasmids - May 2022
TypeBlog Post...efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent... -
All in a Twist: dsRNA
TypeBlog Post...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13 RNA Interference ... -
Guide to Using Pooled Libraries
TypeGuide...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify... -
Addgene's eBook Collection
TypeGuide...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance... -
Science Guides
TypeGuide...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics... -
Developing Lab Management Software for Biology
TypeBlog Post...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post.... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the...