We narrowed to 451 results for: crispr
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TypeBlog Post... In mammalian cells this would be possible with CRISPR/Cas-based genome editing. Selecting a bright fluorescent...
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Visualizing Protein Turnover In Situ
TypeBlog Post...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even... -
All in a Twist: dsRNA
TypeBlog Post...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13 RNA Interference ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic systems ... -
COVID-19 Resources
TypeCollection...may be helpful for COVID-19 research, including: CRISPR tools, like Cas12a and Cas13a plasmids, to reliably... of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: DETECTR (Link opens in a new window... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics... -
Developing Lab Management Software for Biology
TypeBlog Post...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ... -
Botman-Teusink Yeast FP Collection
TypeCollection...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink... -
Synthetic Biology - Overview
TypeCollection...Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit... -
CRISPR Guide
TypeGuide...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology.... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide... -
Plan Your Experiment
TypeGuide... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post.... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...