We narrowed to 452 results for: crispr
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TypeBlog Post...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...
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Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post... RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance... -
Luciferase Plasmid Collection
TypeCollection...enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...Perkins presented a poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You... -
Hot Plasmids February 2024
TypeBlog Post...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ... -
Common Injection Routes in Mice
TypeBlog Post...resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids ... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...others at the Sainsbury are working on a lot of CRISPR tools for editing plant genomes. We will definitely... -
Addgene's eBook Collection
TypeGuide...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ... -
Promega Plasmid Collection
TypeCollection...regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting ... -
Guide to Using Pooled Libraries
TypeGuide...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. The screens... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify... -
Science Guides
TypeGuide...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13... -
Tips for arabidopsis transformation
TypeBlog Post...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...