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We narrowed to 963 results for: tes

Showing: 481 - 500 of 963 results

  1. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...our in-stock service: ddPCR, purity testing, and sterility testing, plus viral genomic sequencing to confirm...AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes or special processes involved; simply request your...

  2. New Viral Vectors - March 2024

    Type
    Blog Post
    ...only March!)  In our repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes...repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New...forgot to use the link below to keep up with the latest in Addgene's viral vectors services and resources...

  4. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...in the United States but around the world. As an organization that directly collaborates with NIH-funded... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious...

  6. CRISPR Guide

    Type
    Guide
    ...interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for... used for small precision edits. NHEJ directly ligates the break ends without the need for a homologous...large genomic deletions or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable...most gRNA targeting sequences will have additional sites of partial homology throughout the genome, called...Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits by copying from a DNA donor...mutation, while whole plasmid or linear dsDNA templates work better for large edits or insertions. When..., where Cas9n nicks the non-target strand and generates a 3’ flap. However, the longer pegRNA also encodes...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...to activate gene transcription. The researchers tested a variety of combinations to optimize both fusion...500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA...synthetase, read this article). The group first tested photocaging various lysines in Cas9 to determine...

  8. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...environmental impact of those processes? Who even regulates them? You may be surprised to learn that it hasn... been 50 years since the creation of the United States Environmental Protection Agency, which started ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...

  9. Reflections and Looking Ahead

    Type
    Blog Post
    ...enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment to open...brings us closer to making an impact on humanity's greatest challenges. Thank you for being part of this incredible...future of science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter...

  10. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...So, as 2013 comes to a close, let’s take a few minutes to reflect on some of the most noteworthy developments...database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from...granted via a public repository or authors’ own web sites. 9)      The year 2013 has also been the year of...

  11. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ... was pumpkin. Everyone gets to vote for their favorites, and trophies are awarded and coveted. I’ve only...we head into Thanksgiving weekend in the United States, I look forward to gathering around a large table...you have any traditions that you share with your labmates, be they food related or not? We would love to...

  12. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ..., M.W., Nguyen, T.H., Coxe, M.A., Miller, B.C., Yates, K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., ..., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations...0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates, K. B., Trombley, J. D., Weiss, S. A., Brown, F....

  13. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...for scientific titles generated from the United States Standard Occupational Classification System  and...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...scientists was Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists...

  14. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... the universal sgRNA is not predicted to target sites in the human, pig, or zebrafish genome, this system... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...

  15. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...to make it more accessible. Other accessibility updates include a printer-friendly design and alt text ...on all images. We have also made accessibility updates to our website, which receives 3.8 million visitors...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

  16. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally...homepage While users are increasingly accessing websites other than through the ‘front door,’ the homepage...Additionally we also wanted to highlight our blog, daily updates, and offer better exploration throughout the site...

  17. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... may appear to be binding at particular genomic sites when in fact they are just non-specific or indirectly... MNase. Once MNase has been localized to target sites, the nuclease is briefly activated to digest the... an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets,...

  19. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression...bacterial cells and selectively grown on antibiotic plates.  Importantly, because the bacteria from which ...

  20. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...used in optogenetic studies are not native to vertebrates and are commonly introduced in zebrafish through... a neural circuit involved in locomotion that generates periodic motor commands for rhythmic movements...  Find plasmids from the Isacoff lab! They then tested what kind of swimming behavior, spontaneous swim...
Showing: 481 - 500 of 963 results